Recent advances in design and application of synthetic membraneless organelles

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2024-04-06 DOI:10.1016/j.biotechadv.2024.108355

Li Wan , Yingying Zhu , Wenli Zhang , Wanmeng Mu

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引用次数: 0

Abstract

Membraneless organelles (MLOs) formed by liquid-liquid phase separation (LLPS) have been extensively studied due to their spatiotemporal control of biochemical and cellular processes in living cells. These findings have provided valuable insights into the physicochemical principles underlying the formation and functionalization of biomolecular condensates, which paves the way for the development of versatile phase-separating systems capable of addressing a variety of application scenarios. Here, we highlight the potential of constructing synthetic MLOs with programmable and functional properties. Notably, we organize how these synthetic membraneless compartments have been capitalized to manipulate enzymatic activities and metabolic reactions. The aim of this review is to inspire readerships to deeply comprehend the widespread roles of synthetic MLOs in the regulation enzymatic reactions and control of metabolic processes, and to encourage the rational design of controllable and functional membraneless compartments for a broad range of bioengineering applications.

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合成无膜细胞器设计与应用的最新进展

通过液-液相分离（LLPS）形成的无膜细胞器（MLOs）因其对活细胞中生化和细胞过程的时空控制而受到广泛研究。这些发现为了解生物分子凝聚物的形成和功能化所依据的物理化学原理提供了宝贵的见解，从而为开发能够应对各种应用场景的多功能相分离系统铺平了道路。在这里，我们将重点介绍构建具有可编程和功能特性的合成 MLO 的潜力。值得注意的是，我们介绍了如何利用这些合成无膜隔室来操纵酶活性和代谢反应。本综述旨在启发读者深刻理解合成 MLO 在调节酶反应和控制代谢过程中的广泛作用，并鼓励合理设计可控的功能性无膜区室，用于广泛的生物工程应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.