Cell membrane vesicles derived from hBMSCs and hUVECs enhance bone regeneration

IF 14.3 1区医学 Q1 CELL & TISSUE ENGINEERING

Bone Research Pub Date : 2024-04-09 DOI:10.1038/s41413-024-00325-9

Dandan Wang, Yaru Guo, Boon Chin Heng, Xuehui Zhang, Yan Wei, Ying He, Mingming Xu, Bin Xia, Xuliang Deng

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Abstract

Bone tissue renewal can be enhanced through co-transplantation of bone mesenchymal stem cells (BMSCs) and vascular endothelial cells (ECs). However, there are apparent limitations in stem cell-based therapy which hinder its clinic translation. Hence, we investigated the potential of alternative stem cell substitutes for facilitating bone regeneration. In this study, we successfully prepared cell membrane vesicles (CMVs) from BMSCs and ECs. The results showed that BMSC-derived cell membrane vesicles (BMSC-CMVs) possessed membrane receptors involved in juxtacrine signaling and growth factors derived from their parental cells. EC-derived cell membrane vesicles (EC-CMVs) also contained BMP2 and VEGF derived from their parental cells. BMSC-CMVs enhanced tube formation and migration ability of hUVECs, while EC-CMVs promoted the osteogenic differentiation of hBMSCs in vitro. Using a rat skull defect model, we found that co-transplantation of BMSC-CMVs and EC-CMVs could stimulate angiogenesis and bone formation in vivo. Therefore, our research might provide an innovative and feasible approach for cell-free therapy in bone tissue regeneration.

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源自 hBMSCs 和 hUVECs 的细胞膜囊泡可促进骨再生

通过联合移植骨间充质干细胞（BMSCs）和血管内皮细胞（ECs），可促进骨组织更新。然而，基于干细胞的疗法存在明显的局限性，阻碍了其临床转化。因此，我们研究了替代干细胞促进骨再生的潜力。在这项研究中，我们成功地制备了BMSCs和ECs的细胞膜囊泡。结果表明，BMSC衍生的细胞膜小泡（BMSC-CMVs）具有参与共刺激信号传导的膜受体和来自亲代细胞的生长因子。EC来源的细胞膜小泡（EC-CMVs）也含有来自亲代细胞的BMP2和血管内皮生长因子。BMSC-CMVs增强了hUVECs的管形成和迁移能力，而EC-CMVs则促进了体外hBMSCs的成骨分化。利用大鼠颅骨缺损模型，我们发现 BMSC-CMVs 和 EC-CMVs 联合移植可刺激血管生成和体内骨形成。因此，我们的研究可能会为骨组织再生中的无细胞疗法提供一种创新而可行的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bone Research CELL & TISSUE ENGINEERING-

CiteScore

20.00

自引率

4.70%

发文量

289

审稿时长

20 weeks

期刊介绍： Established in 2013, Bone Research is a newly-founded English-language periodical that centers on the basic and clinical facets of bone biology, pathophysiology, and regeneration. It is dedicated to championing key findings emerging from both basic investigations and clinical research concerning bone-related topics. The journal's objective is to globally disseminate research in bone-related physiology, pathology, diseases, and treatment, contributing to the advancement of knowledge in this field.

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