Antioxidant activity, cytotoxicity assay, and evaluation of bioactive compounds using GC-MS and HPTLC-based bioassay in the extracts of Osbeckia stellata var. crinita (Benth. ex Naudin) grown in Manipur, India

IF 1.2 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Kuwait Journal of Science Pub Date : 2024-04-04 DOI:10.1016/j.kjs.2024.100229

Tania Baishya , Priya Das , Gouhar Jahan Ashraf , Tarun Kumar Dua , Paramita Paul , Gouranga Nandi , Honey Jajo , Ankita Dutta , Anoop Kumar , Malay Bhattacharya , Ranabir Sahu

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Abstract

In the present study, we aimed to investigate the antioxidant, cytotoxicity activities, and phytochemical profiling of O. stellata extracts. Qualitative phytochemical tests showed the presence of different groups of phytochemicals, mainly in polar solvents. Methanolic and hydroalcoholic extracts showed high total phenolic and flavonoid contents. The antioxidant activity was determined by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), metal chelating, and reducing power assay where methanolic (IC₅₀: 24.76 ± 2.78 μg/mL), hydroalcoholic (IC₅₀: 29.59 ± 3.44 μg/mL), and aqueous (IC₅₀: 33.72 ± 1.97 μg/mL) extracts showed significantly antioxidant potential. Significant cytotoxicity activity was observed in the methanolic extract. As methanolic extract showed better activities, gas chromatography–mass spectrometry (GC-MS) analysis of methanolic extract identified 41 bioactive compounds, where 17 major compounds had medicinal value. High-performance thin layer chromatography (HPTLC) bioassay-guided assay combined with Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) and nuclear magnetic resonance (NMR) spectroscopy characterized gallic and caffeic acid as free radical scavengers. This study evidences that the methanolic extract of O. stellata has potent antioxidant and anti-cancer properties, which may render oxidative stress-related disease and may be used for drug development for human health benefits.

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使用 GC-MS 和基于 HPTLC 的生物分析方法，对印度曼尼普尔省生长的 Osbeckia stellata var.

在本研究中，我们的目的是研究 O. stellata 提取物的抗氧化性、细胞毒性活性和植物化学特征。定性植物化学测试表明，主要在极性溶剂中存在不同类别的植物化学物质。甲醇和水醇提取物显示出较高的总酚和类黄酮含量。使用 2,2-二苯基-1-苦基肼（DPPH）、金属螯合和还原力测定法测定了抗氧化活性，甲醇提取物（IC50：24.76 ± 2.78 μg/mL）、水醇提取物（IC50：29.59 ± 3.44 μg/mL）和水提取物（IC50：33.72 ± 1.97 μg/mL）显示出显著的抗氧化潜力。甲醇提取物具有明显的细胞毒性活性。由于甲醇提取物显示出更好的活性，对甲醇提取物进行气相色谱-质谱（GC-MS）分析，确定了 41 种生物活性化合物，其中 17 种主要化合物具有药用价值。结合衰减全反射-傅立叶变换红外光谱（ATR-FTIR）和核磁共振光谱（NMR）进行的高效薄层色谱法（HPTLC）生物测定指导分析表明，没食子酸和咖啡酸是自由基清除剂。这项研究证明，O. stellata 的甲醇提取物具有很强的抗氧化和抗癌特性，可减少与氧化应激相关的疾病，并可用于药物开发，造福人类健康。

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来源期刊

Kuwait Journal of Science MULTIDISCIPLINARY SCIENCES-

CiteScore

1.60

自引率

28.60%

发文量

132

期刊介绍： Kuwait Journal of Science (KJS) is indexed and abstracted by major publishing houses such as Chemical Abstract, Science Citation Index, Current contents, Mathematics Abstract, Micribiological Abstracts etc. KJS publishes peer-review articles in various fields of Science including Mathematics, Computer Science, Physics, Statistics, Biology, Chemistry and Earth & Environmental Sciences. In addition, it also aims to bring the results of scientific research carried out under a variety of intellectual traditions and organizations to the attention of specialized scholarly readership. As such, the publisher expects the submission of original manuscripts which contain analysis and solutions about important theoretical, empirical and normative issues.