Determination of Phenolics and Flavonoids in Ethanolic Extract of Rosa Centifolia Using UV-Visible Spectroscopy

Natural Volatiles & Essential Oils Journal Pub Date : 2024-02-12 DOI:10.53555/nveo.v11i02.5630

Khushbu Singh

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Abstract

Objective: Our study aimed to analyze the phenolic properties of phytochemicals in ethanolic rose extract (Rosa centifolia L.). UV-Visible spectroscopic methods were used to achieve this goal. UV-Vis spectra helped identify the polyphenols present in the tested extract compared with the literature data. A large correlation was found between the antioxidant potential of the plant material (rose petals) tested. Materials and Methods: The extraction process itself, as well as the processing of rose petals, has undoubtedly significantly reduced the antioxidants present in it. Rose petals were extracted by distillation method using a soxhlet apparatus for 4 hours and fractionated by using various solvent systems, petroleum ether: chloroform, chloroform: ethyl acetate, and ethyl acetate: ethanol of increasing polarity (100:0 to 0:100 with constant polarity increase of 10%) 10 fractions of each solvent system. Results: The ethanolic extract UV-visible spectroscopy profile showed peaks at 268, 270, 285, 401, and 408 nm with the absorption 0.0128, 0.0126, 0.0051, 0.162, and 0.142 respectively. The final fraction of the extract shows 255, 257, 260, 273, 307, 401, and 408 nm with the absorption 0.0190, 0.017, 0.0399, 0.0279, 0.0181, 0.354, and 0.250 respectively. Conclusion: The analysis of the sample shows a noticeable presence of polyphenolic compounds Rose petal extracts can be an alternative to synthetic compounds in cosmetics, food, or medications, such as Trolox, BHA, and BHT.

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利用紫外可见光谱测定百里香乙醇提取物中的酚类和黄酮类化合物

研究目的我们的研究旨在分析玫瑰（Rosa centifolia L.）乙醇提取物中植物化学物质的酚类特性。为此，我们采用了紫外可见光谱法。与文献数据相比，紫外可见光谱有助于确定测试提取物中存在的多酚。结果发现，所测试的植物材料（玫瑰花瓣）的抗氧化潜力之间存在很大的相关性。材料和方法：萃取过程本身以及玫瑰花瓣的加工过程无疑会大大减少其中的抗氧化剂。使用索氏蒸馏器蒸馏法萃取玫瑰花瓣 4 小时，然后使用不同的溶剂系统（石油醚：氯仿、氯仿：乙酸乙酯、乙酸乙酯：乙醇，极性递增（100:0 到 0:100，极性递增 10%））进行分馏，每个溶剂系统 10 个馏分。结果：乙醇提取物紫外可见光谱图显示，在 268、270、285、401 和 408 纳米波长处分别出现吸收峰 0.0128、0.0126、0.0051、0.162 和 0.142。提取物的最终馏分波长分别为 255、257、260、273、307、401 和 408 纳米，吸收率分别为 0.0190、0.017、0.0399、0.0279、0.0181、0.354 和 0.250。结论样品分析表明，玫瑰花瓣提取物中明显含有多酚化合物，可以替代化妆品、食品或药物中的合成化合物，如 Trolox、BHA 和 BHT。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Natural Volatiles & Essential Oils Journal

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