Functionalized gold nanoparticles coated with bacterial alginate and their antibacterial and anticancer activities

IF 3.8 4区工程技术 Q2 CHEMISTRY, MULTIDISCIPLINARY

Green Processing and Synthesis Pub Date : 2024-01-01 DOI:10.1515/gps-2023-0170

Hebah A. Sindi, R. Hamouda, Nuha M. Alhazmi, M. Abdel-Hamid

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Abstract

Gold nanoparticles (Au-NPs) have several uses for nanobiotechnologists because of their beneficial biomedical properties. Alginates have various biomedical and industrial applications. The aim of this study is to extract alginate from Azotobacter chroococcum, synthesize chemical Au-NPs (Ch/Au-NPs), and load the NPs with the extracted alginate to form Azotobacter alginate gold nanocomposites (Azto/Alg-Au-NCMs). The Ch/Au-NPs and Azto/Alg-Au-NCMs were characterized by UV-spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, energy-dispersive spectroscopy (EDS), X-ray diffraction (XRD), thermogravimetric analysis (TGA), zeta potential, and transmission electron microscopy (TEM). The anticancer activities were determined using the breast cancer cell line MCF-7, human lung cancer cell line H1299, and Vero cell line. The results obtained by UV-spectroscopy exhibited a surface plasmon resonance that was clearly noticeable at 530 nm, and the EDS analysis proved that gold was present in percentages of 50.11 and 28.08 in the Ch/Au-NPs and Azto/Alg-Au-NCMs, respectively. There were several similarities between the alginic acid and the alginate extracted from A. chroococcum, and small modifications were proved by FT-IR spectroscopy. Negative charges were shown by the zeta potential. Crystalline and cubic NPs were shown by XRD analysis and TEM. TGA demonstrated the purity of the Ch/Au-NPs and the existence of organic compounds in the Azto/Alg-Au-NCMs. Both the Ch/Au-NPs and Azto/Alg-Au-NCMs had antibacterial activities against Staphylococcus aureus ATCC 25923, Proteus mirabilis, Enterobacter sp., and Pseudomonas aeruginosa and possessed anticancer activities against MCF-7 and H1299.

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涂有细菌藻酸盐的功能化金纳米粒子及其抗菌和抗癌活性

金纳米粒子（Au-NPs）因其有益的生物医学特性而被纳米生物技术专家广泛使用。海藻酸盐具有多种生物医学和工业用途。本研究的目的是从根瘤藻蓝藻中提取藻酸盐，合成化学 Au-NPs （Ch/Au-NPs），并将 NPs 装入提取的藻酸盐，形成根瘤藻藻酸盐金纳米复合材料（Azto/Alg-Au-NCMs）。紫外光谱、傅立叶变换红外光谱、能量色散光谱、X 射线衍射、热重分析、ZETA 电位和透射电子显微镜对 Ch/Au-NPs 和 Azto/Alg-Au-NCMs 进行了表征。使用乳腺癌细胞株 MCF-7、人肺癌细胞株 H1299 和 Vero 细胞株测定了抗癌活性。紫外光谱分析结果表明，在 530 纳米波长处有明显的表面等离子体共振，而 EDS 分析则证明，金在 Ch/Au-NPs 和 Azto/Alg-Au-NCMs 中的含量分别为 50.11 和 28.08%。从 A. chroococcum 中提取的海藻酸和海藻酸盐有几处相似之处，傅立叶变换红外光谱也证明了它们的细微变化。zeta 电位显示了负电荷。XRD 分析和 TEM 显示了结晶和立方 NPs。TGA 证明了 Ch/Au-NPs 的纯度以及 Azto/Alg-Au-NCMs 中有机化合物的存在。Ch/Au-NPs 和 Azto/Alg-Au-NCMs 对金黄色葡萄球菌 ATCC 25923、奇异变形杆菌、肠杆菌和铜绿假单胞菌具有抗菌活性，对 MCF-7 和 H1299 具有抗癌活性。

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来源期刊

Green Processing and Synthesis CHEMISTRY, MULTIDISCIPLINARY-ENGINEERING, CHEMICAL

CiteScore

6.70

自引率

9.30%

发文量

审稿时长

7 weeks

期刊介绍： Green Processing and Synthesis is a bimonthly, peer-reviewed journal that provides up-to-date research both on fundamental as well as applied aspects of innovative green process development and chemical synthesis, giving an appropriate share to industrial views. The contributions are cutting edge, high-impact, authoritative, and provide both pros and cons of potential technologies. Green Processing and Synthesis provides a platform for scientists and engineers, especially chemists and chemical engineers, but is also open for interdisciplinary research from other areas such as physics, materials science, or catalysis.