CHANGES IN THE EXPRESSION OF LONG NON-CODING RNAS H19, TUG1, GAS5, MIAT DURING MYOCARDIAL ISCHEMIA-REPERFUSION

Fiziolohichnyĭ zhurnal Pub Date : 2024-01-01 DOI:10.15407/fz70.01.052

M. Khetsuriani, T. I. Drevytska, A. M. Shysh

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Abstract

Long non-coding RNAs (lncRNAs) are the most numerous group of transcripts performing various functions, including the development of cardiovascular pathologies. We investigated the changes in expression of four long non-coding RNAs (H19, TUG1, GAS5, MIAT) under conditions of anoxia-reoxygenation in neonatal rat cardiomyocyte culture and ischemia-reperfusion in adult Wistar rats. A significant decrease in the expression of all four long non-coding RNAs in cell culture under experimental conditions was established. The regime of prolonged anoxia-reoxygenation led to a sharp increase in the expression level of long non-coding RNA MIAT by twice, but compared to normoxia, these changes were not significant. After ischemia-reperfusion in rat myocardium, the content of long non-coding RNA TUG1 increased by 22 times, while the expression of H19 decreased by 3.79 times, and in rat plasma, the expression of long non-coding RNA MIAT increased by 3.79 times. The obtained results allow considering long non-coding RNAs H19 and TUG1 as potential targets in ischemic myocardial injury, and MIAT as a biomarker of cardiovascular pathologies.

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心肌缺血再灌注过程中长非编码 rnas h19、tug1、gas5 和 miat 的表达变化

长非编码 RNA（lncRNA）是数量最多的一组转录本，具有多种功能，包括心血管病变的发生。我们研究了四种长非编码 RNA（H19、TUG1、GAS5、MIAT）在新生大鼠心肌细胞培养缺氧-再缺氧和成年 Wistar 大鼠心肌细胞培养缺血-再灌注条件下的表达变化。在实验条件下，所有四种长非编码 RNA 在细胞培养中的表达均明显下降。长时间缺氧-再缺氧导致长非编码 RNA MIAT 的表达水平急剧增加了一倍，但与常氧相比，这些变化并不显著。大鼠心肌缺血再灌注后，长非编码 RNA TUG1 的含量增加了 22 倍，而 H19 的表达量减少了 3.79 倍；在大鼠血浆中，长非编码 RNA MIAT 的表达量增加了 3.79 倍。研究结果表明，长非编码 RNA H19 和 TUG1 是缺血性心肌损伤的潜在靶点，而 MIAT 则是心血管病变的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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