UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN RAMANIA (Bouea macrophylla Griffith) TERHADAP BAKTERI Staphylococcus aureus

Abstract

The ramania plant (Bouea macrophylla Griffith) is a species from the Anacardiaceae family, with different names. The use of ramania plants (Bouea macrophylla Griffith) is still very limited, namely only as fruit. Empirically, ramania leaves (Bouea macrophylla Griffith) have the potential to control the wound healing process. The aim of this research was to determine the results of phytochemical screening and the antibacterial activity of 96% ethanol extract of Ramania leaves (Bouea macrophylla Griffith) against Staphylococcus aureus bacteria. The research method uses the disc method with 4 series of 5% concentration; 10%; 15% and 20%, positive control chloramphenicol 30μg/disc, and negative control DMSO 0.1%. The results of this study showed that the 96% ethanol extract of Ramania leaves (Bouea macrophylla Griffith) was positive for containing alkoloids, flavonoids, steroids, saponins and tannins using the maceration method using 96% ethanol solvent and had an extract yield of 8.54%. Antibacterial activity test at concentrations of 5%, 10%, 15% and 20% with results and inhibition zone categories of 1,925 mm (weak), 3,355 mm (weak), and 4,737 mm (weak) and 5,775 mm (medium) respectively. The conclusion of the research shows that ethanol extract from 96% ramania leaves (Bouea macrophylla Griffith) has antibacterial activity against Staphylococcus aureus bacteria.