Ultrastructural Changes in Thyroid Gland from Fetus and Maternal Rats Rattus Norvegicus with Induced Hypothyroidism

Anwar N. Sewan, Maha Ibrahim, Alaa Hussein
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Abstract

The study evaluated the effect of hypothyroidism on experimental rats (Rattus norvegicus). All animals were housed, breeding and adapted, and then only (50) virgin females were chosen. The animals subdivided into two groups, the first group was regarded as control, while the second group were treated with propylthiouracil (ptu) as a drug with a dose (0.05%) to induce hypothyroidism, then the females left mating and studied the changes after (14.5,16.5,18.5,20.5and 21.5) days post gestation. In this study, the results of ultra-structural changes in the thyroid gland from maternal rats and fetus with induced hypothyroidism and their fetus at each period of gestation were clarified with transmission electron microscopy; these changes included alteration in apical surface and cytoplasm of follicular cells that lining the thyroid follicles by Changes in shapes of microvilli. Like blebs and protrusions, also dilated rough endoplasmic reticulum, increased with lysosomes, and the empty vacuoles appeared more than the dense vesicles. In addition to that, there was an increase in mitochondria and golgi apparatus cisternae, the nucleus of follicular cell showed changes in their chromatin and irregular outlines and more cellular debris. Also, the pictures referred to the deposition of crystals from iodine salts in most follicular cells and the connective tissue showed thickening that separated thyroid follicles from rat fetus with induced hypothyroidism at (18.5) days post gestation. Biochemical results revealed to a significant increase in the mean concentration of (TSH)in all pregnant rats with hypothyroidism compared with control females, also there were a decreased in both (T3 and T4) hormones in the serum of pregnant rats in the hypothyroidism group compared to the control group, during each period of gestation (14.5,16.5,18.5,20.5 and 21.5) days. Levels of some oxidative enzymes (GSH and MDA) were also determined in this study; there was a significant increase in their concentration in serum of pregnant rats related to the hypothyroidism group compared with their concentration in the control group.
诱发甲状腺功能减退症的胎鼠和母鼠甲状腺的超微结构变化
该研究评估了甲状腺机能减退对实验鼠(Rattus norvegicus)的影响。对所有动物进行饲养、繁殖和适应,然后只选择(50 只)雌性处女。将动物分成两组,第一组为对照组,第二组使用剂量为 0.05% 的丙基硫氧嘧啶(ptu)诱导甲状腺机能减退,然后雌鼠停止交配,研究妊娠后(14.5、16.5、18.5、20.5 和 21.5)天后的变化。在这项研究中,透射电子显微镜明确了甲状腺功能减退症母鼠和甲状腺功能减退症胎儿在妊娠各个时期的甲状腺超结构变化结果;这些变化包括甲状腺滤泡内膜滤泡细胞顶端表面和细胞质的改变,以及微绒毛形状的改变。此外,粗面内质网扩张,溶酶体增多,空泡多于致密泡。此外,线粒体和高尔基体空泡增多,滤泡细胞核的染色质发生变化,轮廓不规则,细胞碎片增多。此外,图片还显示,大多数卵泡细胞中都有碘盐结晶沉积,结缔组织增厚,使妊娠后(18.5)天的甲状腺诱导性甲状腺功能减退症大鼠胎儿的甲状腺滤泡分离。生化结果显示,与对照组雌性大鼠相比,所有甲状腺功能减退症妊娠大鼠的平均促甲状腺激素(TSH)浓度都明显升高;与对照组相比,甲状腺功能减退症组妊娠大鼠在妊娠14.5、16.5、18.5、20.5和21.5天期间血清中的T3和T4激素都有所下降。本研究还测定了一些氧化酶(GSH和MDA)的水平;与对照组相比,甲状腺机能减退组孕鼠血清中这些酶的浓度显著增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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