Biochemical characterization of bacteria isolated from Rhizosphere soils of sugarcane grown in Uttar Pradesh

Abstract

Rhizospheric soil samples of Sugarcane fields were collected from distinct regions of U.P. Further, these soil samples were used for the isolation and characterization of bacteria to identify their maximum PGPR traits and biochemical potential. A total of ten bacterial isolates were collected from the soil samples and sub-cultured in the nutrient agar plates and characterized through Biochemical test. One bacterial strain was found negative and the other 9 isolates were found positive for gram staining. All bacterial isolates were positive for citrate test. 3 isolates were found MR positive and 2 positives for VP test. 5 isolates were positive for gelatinase test and 9 isolates were positive for nitrate reductase test. 2 isolates were positive for starch hydrolysis test, while 7 isolates were found positive for phosphate solubilization test and 3 isolates were positive for siderophore production test. These results showed that the bacterial isolates from soil sample were Azotobacter Species, Azospirillum Species, Pseudomonas species, Bacillus Species, Rhizobium Species, Serratia Species and E. coli. These bacterial strains followed the maximum PGPR traits. After proper validation, these bacterial strains could be used for the bio-fertilizer formation for crop production enhancement by improving soil fertility.