ECOLOGICAL AND HYGIENIC ASSESSMENT OF THE QUALITY OF BURDOCK ROOTS OF LARGE FLORA OF URBANIZED TERRITORIES OF THE CENTRAL BLACK EARTH REGION

N. Dyakova
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Abstract

The roots of the bladder are mainly used in the form of aqueous extracts, and the pharmacological effect is due to water-soluble compounds based on polysaccharides. The study of the peculiarities of accumulation of this group of compounds is relevant. The aim of this study the relationship between the accumulation of heavy metals and arsenic and biologically active substances in the roots of ordinary burdock, collected on the territory of the Voronezh region in biogeocenoses experiencing various anthropogenic effects. In the Voronezh region, 51 points of harvesting plant raw materials were selected. The study of the content of heavy metals and arsenic in the roots of the bladder, the sum of polysaccharides in terms of fructose and the sum of extractive substances extracted by water was conducted according to pharmacopeic methods. The total amount of gravimetrically precipitated water-soluble polysaccharides was determined by a previously developed and validated patented procedure. Correlation coefficients were analyzed to examine in detail the effects of heavy metals and arsenic on the accumulation of biologically active substances. Of the samples examined, some do not meet the requirements of the regulatory documentation for arsenic content. Significant physiological barriers have been identified to the accumulation of ecotoxicants in the roots of common burdock, which is especially noticeable for elements such as lead, mercury, arsenic, cadmium, cobalt and chromium. The raw material can selectively concentrate copper and zinc. In eight samples prepared under urbocenosis conditions, this indicator was below the minimum numerical value. Low correlation of the content of large sum of gravimetrically determined polysaccharides and extractive substances in samples of burdock roots was revealed. extracted with water, containing heavy metals and arsenic, wherein the numerical value of the sum of the polysaccharides in terms of fructose is largely dependent on the concentration of toxic elements in the plant; which is probably due to the formation of strong metal-monosaccharide complexes, which make it difficult to obtain colored complexes of monomer sugars with a complexing agent during spectrophotometric determination.
黑土地中部城市化地区大型植物区系牛蒡根质量的生态和卫生评估
膀胱根主要以水提取物的形式使用,其药理作用归功于以多糖为基础的水溶性化合物。研究这组化合物积累的特殊性具有现实意义。本研究的目的是研究在沃罗涅日地区境内遭受各种人为影响的生物地理环境中采集的普通牛蒡根中重金属和砷的积累与生物活性物质之间的关系。在沃罗涅日州选择了 51 个植物原料采摘点。根据药典方法,对膀胱根中的重金属和砷含量、以果糖计的多糖总和以及用水提取的萃取物质总和进行了研究。水溶性多糖的重力沉淀总量是通过先前开发和验证的专利程序测定的。分析了相关系数,以详细研究重金属和砷对生物活性物质积累的影响。在检测的样品中,有些不符合砷含量监管文件的要求。研究发现,普通牛蒡根部的生态毒物积累存在明显的生理障碍,尤其是铅、汞、砷、镉、钴和铬等元素。这种原料可以选择性地浓缩铜和锌。在城市化条件下制备的 8 个样本中,该指标低于最低数值。在用水提取的含有重金属和砷的牛蒡根样本中,用重量测定法测定的多糖总和与萃取物质的含量相关性较低,其中以果糖计的多糖总和的数值在很大程度上取决于植物中有毒元素的浓度;这可能是由于形成了强烈的金属-单糖复合物,使得在分光光度测定过程中很难用络合剂获得单体糖的有色络合物。
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