Effect of Some Metals Ions on Hemolysin Production from Clinical Isolates of Escherichia Coli

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL

Journal of Contemporary Medical Sciences Pub Date : 2024-02-26 DOI:10.22317/jcms.v10i1.1450

Eman Khadum Juda, K. J. Khalaf

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Abstract

Objective:This study aimed to determine effect of some ions metals (Ca+2, Mn+2, Mg+2, Fe+2, K+, Na+, Zn+2) on hemolysin production was performed. Methods: Eighty Three (83) E. coliisolates collected from different clinical sources from Baghdad Hospitals, performed by using cultural traits, morphological features and biochemical tests, confirmation of identification was done by Vitek 2 system.And it was detected on ability these isolates to production hemolysin by two methods liquid media and agar media. Results: The results showed that: In agar medium revealed that 6 (7.23 %) isolates of E. coli had the ability for producing this enzyme and 77 (92.77%) did not produce hemolysin. The same results were found in liquid medium. Used the microtitration plate method to study the effect these ions metals on hemolysin production.According to the effect of ions metals, the results also showed that MIC value of ions metals was 500μg/mL, hemolysin production was increased after addition each of(Ca+2,Mn+2, Mg+2,Fe+2,K+,Na+) the percentage of hemolysis (96.0 %, 93.4 %, 93.2 %, 93.3%,94.2%, 93.7%) respectively while hemolysin production decreased after addition of Zn+2 with the percentage of hemolysis 0.3%. Conclusion: Based on the influence of metal ions, the findings indicated that hemolysin production exhibited an increase following the addition of each of Ca2+, Mn2+, Mg2+, Fe2+, K+, and Na+ when compared to the control group. Conversely, the introduction of Zn2+ led to a decrease in hemolysin production.

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某些金属离子对临床大肠杆菌分离株溶血素产生的影响

目的：本研究旨在确定一些金属离子（Ca+2、Mn+2、Mg+2、Fe+2、K+、Na+、Zn+2）对溶血素产生的影响：方法：从巴格达医院的不同临床来源收集了 83 株大肠埃希氏菌，利用培养特性、形态特征和生化测试对其进行鉴定，并通过 Vitek 2 系统进行确认：结果表明在琼脂培养基中，有 6 株（7.23%）大肠杆菌具有产生溶血素的能力，77 株（92.77%）大肠杆菌不产生溶血素。在液体培养基中也发现了同样的结果。使用微滴定板法研究这些金属离子对溶血素产生的影响。根据金属离子的影响，结果还显示，金属离子的 MIC 值为 500μg/mL，加入 Ca+2、Mn+2、Mg+2、Fe+2、K+、Na+ 后，溶血素产生的百分比增加（96.0 %、93.4 %、93.4 %、96.0 %）。0%、93.4%、93.2%、93.3%、94.2%、93.7%），而加入 Zn+2 后溶血素产量下降，溶血率为 0.3%：根据金属离子的影响，研究结果表明，与对照组相比，添加 Ca2+、Mn2+、Mg2+、Fe2+、K+ 和 Na+ 后，溶血素的产生量增加。相反，添加 Zn2+ 则导致溶血素产生量减少。

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来源期刊

Journal of Contemporary Medical Sciences MEDICINE, GENERAL & INTERNAL-

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审稿时长

12 weeks