Prospective hepatotoxic effect of UV-328 and its affirmable rescue by Dimethoxy curcumin in Zebrafish

Abstract

The unprecedented usage of BUV-328 (Benzotriazole Ultraviolet Stabilizer) in many biological and environmental matrices is of acute environmental importance because of its toxicity even at low concentrations. To better understand the protective function of DiMC on the liver tissues of zebrafish exposed to sublethal concentration of BUV-328 was assessed in the present investigation. Adult zebrafish were exposed to BUV-328 at sublethal concentrations of 55µg/l. The responses were assessed in the liver tissues at 28 days and another group was supplemented with DiMC to investigate its ameliorative potential against BUV-328 induced hepatotoxicity. Biochemical markers of oxidative stress, histopathological changes, and antioxidant enzymes were measured in the exposed groups. The outcomes of our present study revealed that BUV-328 exposure upregulated the oxidative stress markers and diminished the activities of the antioxidant enzymes, altered the biochemical constituents in liver. Histopathological abrasions such as hypertrophy, cellular and nuclear enlargement, cytoplasmic and nuclear degeneration, necrosis with pyknotic nuclei, lipid and cytoplasmic vacuolization and nuclear displacement to the periphery were found to be increased in BUV-328 exposure group. The oxidative, biochemical and histological alterations induced by BUV-328 were almost recuperated in DiMC supplemented group which signifies its protective influence against BUV-328 incited hepatotoxicity.