Isolation and Identification of Biofilm forming Bacteria from Glass Panels exposed in Sea Waters of South Andaman, India

IF 0.2 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Shehin M.C. Sadaka, K.A. Jayaraj, Ameen Ummath, Venu Sasidharan, Fahmeeda P.S. Parveen
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引用次数: 0

Abstract

Marine organisms attach to surfaces that are submerged in seawater in a process known as biofouling. These organisms can colonize on various surfaces such as ship hulls, pillars, jetties, undersea pipelines, oil rigs and buoys. Foulers can include bacteria, algae, barnacles, mussels and other invertebrates. The initial adhesion of fouling bacteria is the first stage of biofouling. The growth and attachment of marine fouling bacteria are influenced by various environmental factors including salinity, temperature and nutrient availability. The bacteria forming marine biofilm were isolated and identified from glass panels. Basic microbiological phenotypic identification methods and genotypic validation using 16s rRNA gene sequencing were used to identify the bacterial strains. The phylogenetic analysis of the 16s rRNA gene sequences confirmed 13 different species (Bacillus aquimaris (2), Vibrio hepatarius (1), Exiguobacterium profundum (3), Exiguobacterium aestuarii (2), Vibrio parahaemolyticus (2), Vibrio alginolyticus (3), Vibrio azureus (2), Vibrio fluvialis (2), Priestia flexa (1), Pseudomonas aeruginosa (2), Bacillus megaterium (2), Lysinibacillus macroides (2) and Bacillus subtilis (1)) of fouling bacteria belonging to six genera.
从暴露在印度南安达曼海水中的玻璃板上分离和鉴定形成生物膜的细菌
海洋生物附着在浸没在海水中的表面,这一过程被称为生物污损。这些生物可在船体、支柱、防波堤、海底管道、石油钻井平台和浮标等各种表面上定居。污损生物包括细菌、藻类、藤壶、贻贝和其他无脊椎动物。污损细菌的最初附着是生物污损的第一阶段。海洋污损细菌的生长和附着受各种环境因素的影响,包括盐度、温度和营养供应。从玻璃板上分离并鉴定了形成海洋生物薄膜的细菌。采用基本的微生物表型鉴定方法和 16s rRNA 基因测序的基因型验证方法来鉴定细菌菌株。藻溶性弧菌 (3)、天蓝色弧菌 (2)、河水弧菌 (2)、柔藻弧菌 (1)、铜绿假单胞菌 (2)、巨大芽孢杆菌 (2)、大肠溶血芽孢杆菌 (2) 和枯草芽孢杆菌 (1))。
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来源期刊
Research Journal of Biotechnology
Research Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
0.60
自引率
0.00%
发文量
192
期刊介绍: We invite you to contribute Research Papers / Short Communications / Review Papers: -In any field of Biotechnology, Biochemistry, Microbiology and Industrial Microbiology, Soil Technology, Agriculture Biotechnology. -in any field related to Food Biotechnology, Nutrition Biotechnology, Genetic Engineering and Commercial Biotechnology. -in any field of Biotechnology related to Drugs and Pharmaceutical products for human beings, animals and plants. -in any field related to Environmental Biotechnolgy, Waste Treatment of Liquids, Soilds and Gases; Sustainability. -in inter-realted field of Chemical Sciences, Biological Sciences, Environmental Sciences and Life Sciences. -in any field related to Biotechnological Engineering, Industrial Biotechnology and Instrumentation. -in any field related to Nano-technology. -in any field related to Plant Biotechnology.
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