EXTRACELLULAR EXPRESSION OF THE ALPHA-AMYLASE GENE FROM BACILLUS AMYLOLIQUEFACIENS MDC1974 STRAIN USING BACILLUS SUBTILIS RIK1285 CELLS

Abstract

The alpha-amylase gene from Bacillus amyloliquefaciens MDC1974 strain was molecularly cloned into the E. coli/B. subtilis pBE-S shuttle vector and subsequently expressed extracellularly by the recipient strain Bacillus subtilis RIK1285, which exhibited low protease activity. As a result of optimizing fermentation conditions, a secretion of alpha-amylase with an activity of 1400 units per mL was achieved.