The Protection of Enzyme Activity for the Preparation of Humanized Polymerized Hemoglobin-Superoxide Dismutase-Catalase-Carbonic Anhydrase

IF 0.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2024-03-27 DOI:10.1134/S1607672923600483

Yaoxi Wang, Wanjun Wang, Lili Zhang, Gang Chen

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Abstract

This work finds suitable enzyme activity protectants to improve the recovery rate of enzyme activity in the preparation of human polymerized hemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA), including trehalose, sucrose, glucose, hydroxypropyl-β-cyclodextrin, and mannitol.Different types and concentrations of enzyme activity protective agents were added during polymerization to compare their protective ability to enzyme activity and the effect on the properties of hemoglobin. The study found that compared with trehalose, the protective effect of sucrose on CA enzyme activity is non-significant to that on hemoglobin, the recovery rate of SOD, and CAT enzyme activity has significant increased. Glucose, hydroxypropyl-β-cyclodextrin, and mannitol are unsuitable for the added enzyme activity protective agent of PolyHb-SOD-CAT-CA.The protective effect of sucrose on CA was non-significant with trehalose. The protective effect of sucrose on SOD and CAT enzyme activity was higher than trehalose, and the protective effect reached the maximum when the concentration reached 1.5%.

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制备人源化聚合血红蛋白-超氧化物歧化酶-催化酶-碳酸酐酶的酶活性保护。

本研究在制备人聚合血红蛋白-超氧化物歧化酶-催化酶-碳酸酐酶（PolyHb-SOD-CAT-CA）的过程中，发现了合适的酶活性保护剂，包括树胶糖、蔗糖、葡萄糖、羟丙基-β-环糊精和甘露醇，在聚合过程中加入不同种类和浓度的酶活性保护剂，比较其对酶活性的保护能力和对血红蛋白性质的影响。研究发现，与三卤糖相比，蔗糖对 CA 酶活性的保护作用对血红蛋白的保护作用不显著，SOD、CAT 酶活性的恢复率显著提高。葡萄糖、羟丙基-β-环糊精和甘露醇不适合作为 PolyHb-SOD-CAT-CA 的酶活性保护剂。蔗糖对 SOD 和 CAT 酶活性的保护作用高于树胶糖，当浓度达到 1.5%时，保护作用达到最大。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.