Hyogu Han, Yang Chan Park, Kwang-Kyu Kim, Hak Ju Kim, Han Kyu Seo, Jeanho Park, Jae Sun Moon, Jun Ki Ahn
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引用次数: 0
Abstract
This study presents a novel method for detecting plant viruses by combining a personal glucose meter (PGM)-based cascade enzymatic reaction (CER) with loop-mediated isothermal amplification (LAMP). This technique exploits the consumption of deoxynucleotides (dNTPs) during the LAMP process as a substrate for CER, leading to a measurable change in glucose concentration. This change can be detected using PGM, enabling the identification of the presence or absence of the target virus. This method provide a more efficient alternative to traditional methods like ELISA and PCR. It overcomes their limitation in terms of laboratory equipment requirement, sensitivity, and on-site applicability. In addition, we also developed a portable diagnostic device that integrates a heating block with a glucose measurement module. By utilizing this device, the rapid and precise detection of various plant viruses, including horseradish latent virus (HRLV), onion yellow dwarf virus (OYDV), soybean yellow common mosaic virus (SYCMV), cnidium vein yellowing virus 1 (CnVYV-1), and perilla mosaic virus (PerMV), successfully achieved within 40 min. This advancement offers a practical and cost-effective solution for managing plant pathogen threats in agriculture.
期刊介绍:
BioChip Journal publishes original research and reviews in all areas of the biochip technology in the following disciplines, including protein chip, DNA chip, cell chip, lab-on-a-chip, bio-MEMS, biosensor, micro/nano mechanics, microfluidics, high-throughput screening technology, medical science, genomics, proteomics, bioinformatics, medical diagnostics, environmental monitoring and micro/nanotechnology. The Journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.