Human IgE-FcεRI chip assay to screen natural products for antipruritic activity

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research Pub Date : 2024-03-21 DOI:10.1016/j.sbsr.2024.100640

Yong Wan Cho , Ik Soo Lee , Hye-Won Lim , Young Sook Kim

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引用次数: 0

Abstract

A biochip assay provides high-throughput and multiplexed analysis of biological samples, chemicals, and natural products. Pruritus is itchiness due to several causes, such as allergy, dry skin, pregnancy, liver disease, kidney disease, and thyroid disease. Treatment of pruritus is associated with reduced immunoglobulin (IgE) and FcεRI levels in sera. Treatment of pruritus include corticosteroid creams or ointment. However, they have side effects, so we need a safer treatment using natural products. Here, we developed an assay using protein chip technology to identify natural products with antipruritic activity that inhibit IgE-FcεRI binding. Of the 28 tested natural product extracts, Corni Fructus extract inhibited human IgE-FcεRI binding and also showed anti-histamine effects in MC/9 mast cells. These results suggest that this protein biochip assay system can be used to identify promising natural product extracts for the treatment of pruritus.

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人 IgE-FcεRI 芯片测定法筛选具有止痒活性的天然产品

生物芯片分析法可对生物样本、化学品和天然产品进行高通量和多重分析。瘙痒症是由多种原因引起的瘙痒，如过敏、皮肤干燥、妊娠、肝脏疾病、肾脏疾病和甲状腺疾病。瘙痒症的治疗与血清中免疫球蛋白（IgE）和 FcεRI 水平的降低有关。瘙痒症的治疗包括使用皮质类固醇药膏或软膏。然而，这些药物都有副作用，因此我们需要使用天然产品进行更安全的治疗。在此，我们利用蛋白芯片技术开发了一种检测方法，以鉴定具有抑制 IgE-FcεRI 结合的抗瘙痒活性的天然产品。在测试的 28 种天然产品提取物中，柯尼果提取物可抑制人 IgE-FcεRI 结合，并在 MC/9 肥大细胞中显示出抗组胺作用。这些结果表明，这种蛋白质生物芯片检测系统可用于鉴别治疗瘙痒症的有前景的天然产品提取物。

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来源期刊

Sensing and Bio-Sensing Research Engineering-Electrical and Electronic Engineering

CiteScore

10.70

自引率

3.80%

发文量

审稿时长

87 days

期刊介绍： Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies. The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.