PPP2R2A promotes Hu sheep pituitary cell proliferation and gonadotropin secretion associated with prolificacy

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2024-03-13 DOI:10.1016/j.anireprosci.2024.107457

Hui Xu , Yu Cai , Hua Yang , Shanglai Li , Peiyong Chen , Zongyou Wei , Feng Wang , Ziyu Wang , Yanli Zhang

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Abstract

The anterior pituitary plays a critical role in the endocrine system, contains gonadotrophs, which regulate reproductive efficiency by secreting follicle-stimulating hormone (FSH) and luteinizing hormone (LH). PPP2R2A is a serine-threonine phosphatase that regulates reproductive functions in both females and males, its function in pituitary cells remain unclear. Hu sheep is a highly prolific breed, which makes it suitable for studying reproductive mechanisms. In this study, the relative abundances of PPP2R2A mRNA expression were higher in the pituitary of high-prolificacy (HF) Hu sheep compared to those of low-prolificacy (LF) Hu sheep. Additionally, we demonstrated that PPP2R2A promotes pituitary cell proliferation and gonadotropin secretion using the EdU assay and ELISA, respectively. Moreover, it inhibits pituitary cell apoptosis using flow cytometry. Furthermore, PPP2R2A may affect pituitary cell function by regulating the AKT/mTOR signaling pathway. In summary, our findings suggest that PPP2R2A may play a role in regulating pituitary function and influencing the secretion of gonadotropins.

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PPP2R2A 促进绵羊垂体细胞增殖和促性腺激素分泌

垂体前叶在内分泌系统中发挥着关键作用，它含有促性腺激素，通过分泌卵泡刺激素（FSH）和黄体生成素（LH）来调节生殖效率。PPP2R2A 是一种丝氨酸-苏氨酸磷酸酶，可调节雌性和雄性的生殖功能，但它在垂体细胞中的功能仍不清楚。胡羊是一个高产品种，因此适合研究其生殖机制。在这项研究中，与低繁殖力（LF）胡羊相比，高繁殖力（HF）胡羊垂体中 PPP2R2A mRNA 表达的相对丰度更高。此外，我们还利用 EdU 试验和酶联免疫吸附试验分别证明了 PPP2R2A 能促进垂体细胞增殖和促性腺激素分泌。此外，它还通过流式细胞术抑制了垂体细胞的凋亡。此外，PPP2R2A 可能通过调节 AKT/mTOR 信号通路来影响垂体细胞的功能。总之，我们的研究结果表明，PPP2R2A 可能在调节垂体功能和影响促性腺激素分泌方面发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.

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