{"title":"Trehalose-induced metabolic responses in\nbasil (Ocimum basilicum) seedlings under salt treatment","authors":"Ramazan Karamzehi, A. Einali","doi":"10.37427/botcro-2024-021","DOIUrl":null,"url":null,"abstract":"Trehalose (Tre) is an osmoprotectant known to be an important player in regulating response to salinity. In this research, the effect of Tre foliar application on the growth rate, the content of photosynthetic pigments, accumulation of metabolites, and activity of antioxidant enzymes of basil seedlings under salt stress has been investigated. Basil seedlings were factorially treated twice a week with levels of 0, 25, 50, 100, and 150 mM NaCl and weekly with concentrations of 0 and 5 mM Tre for 4 weeks. Growth characteristics, the content of photosynthetic pigments including chlorophyll and carotenoid, and starch content decreased in response to salt stress, while the activity of antioxidant enzymes and the accumulation of metabolites including soluble sugars, proteins, amino acids, and proline increased. Tre treatment caused severe inhibition of plant growth, further reduction of photosynthetic pigments, and amount of soluble proteins during salinity stress. Also, starch, total protein, amino acids, and proline were hyperaccumulated in response to Tre. These results indicate that Tre not only does not reduce the detrimental effects of salinity in basil seedlings but also inhibits plant growth possibly by diverting carbon to other metabolic pathways.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Botanica Croatica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.37427/botcro-2024-021","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Trehalose (Tre) is an osmoprotectant known to be an important player in regulating response to salinity. In this research, the effect of Tre foliar application on the growth rate, the content of photosynthetic pigments, accumulation of metabolites, and activity of antioxidant enzymes of basil seedlings under salt stress has been investigated. Basil seedlings were factorially treated twice a week with levels of 0, 25, 50, 100, and 150 mM NaCl and weekly with concentrations of 0 and 5 mM Tre for 4 weeks. Growth characteristics, the content of photosynthetic pigments including chlorophyll and carotenoid, and starch content decreased in response to salt stress, while the activity of antioxidant enzymes and the accumulation of metabolites including soluble sugars, proteins, amino acids, and proline increased. Tre treatment caused severe inhibition of plant growth, further reduction of photosynthetic pigments, and amount of soluble proteins during salinity stress. Also, starch, total protein, amino acids, and proline were hyperaccumulated in response to Tre. These results indicate that Tre not only does not reduce the detrimental effects of salinity in basil seedlings but also inhibits plant growth possibly by diverting carbon to other metabolic pathways.
据了解,海藻糖(Trehalose,Tre)是一种渗透保护剂,在调节盐度反应方面起着重要作用。本研究探讨了叶面喷施 Tre 对盐胁迫下罗勒幼苗的生长速率、光合色素含量、代谢产物积累和抗氧化酶活性的影响。罗勒幼苗每周接受两次浓度分别为 0、25、50、100 和 150 mM NaCl 的因素处理,每周接受一次浓度分别为 0 和 5 mM Tre 的因素处理,持续 4 周。生长特性、光合色素(包括叶绿素和类胡萝卜素)含量和淀粉含量在盐胁迫下均有所下降,而抗氧化酶的活性和代谢产物(包括可溶性糖、蛋白质、氨基酸和脯氨酸)的积累则有所增加。在盐胁迫期间,Tre 处理严重抑制了植物的生长,进一步降低了光合色素和可溶性蛋白质的含量。此外,淀粉、总蛋白、氨基酸和脯氨酸也在 Tre 的作用下过度积累。这些结果表明,Tre 不仅不能减轻盐度对罗勒幼苗的不利影响,还可能通过将碳转移到其他代谢途径而抑制植物生长。
期刊介绍:
The interest of the journal is field (terrestrial and aquatic) and experimental botany (including microorganisms, plant viruses, bacteria, unicellular algae), from subcellular level to ecosystems. The attention of the Journal is aimed to the research of karstic areas of the southern Europe, karstic waters and the Adriatic Sea (Mediterranean).