CgPHB2 involved in the haemocyte mitophagy in response to Vibrio splendidus stimulation in Pacific oyster Crassostrea gigas

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Shurong Li , Jiejie Sun , Yinan Li , Xiaoqian Lv , Lingling Wang , Linsheng Song
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Abstract

Prohibitin2 (PHB2) is recently identified as a novel inner membrane mitophagy receptor to mediate mitophagy. In the present study, the function of CgPHB2 in mediating mitophagy in response to Vibrio splendidus stimulation was investigated in Crassostrea gigas. CgPHB2 protein was mainly distributed in the cytoplasm of three subpopulations of haemocytes. After V. splendidus stimulation, the expressions of CgPHB2 mRNA in haemocytes were up-regulated significantly at 6, 12 and 24 h, and the abundance of CgPHB2 protein was also enhanced at 12–24 h compared to control group. Furthermore, the green signals of CgPHB2 were colocalized respectively with the red signals of mitochondria and CgLC3 in the haemocytes at 12 h after V. splendidus stimulation, and the co-localization value of CgPHB2 and mtphagy Dye was significantly increased. The direct interaction between CgPHB2 and CgLC3 was simulated by molecular docking. In PHB2-inhibitor Fluorizoline-treated oysters, the mRNA expressions of mitophagy-related genes and the ratio of mitophagy were significantly decreased in haemocytes of oysters after V. splendidus stimulation. All the results collectively suggested that CgPHB2 participated in mediating the haemocyte mitophagy in the antibacterial immune response of oysters.

CgPHB2 参与了太平洋巨牡蛎在绚丽弧菌刺激下的血细胞有丝分裂。
最近发现抑制素2(PHB2)是一种新型内膜有丝分裂受体,可介导有丝分裂。本研究探讨了 CgPHB2 在绚丽弧菌刺激下介导巨尾鲈有丝分裂的功能。CgPHB2 蛋白主要分布在三个亚群血细胞的细胞质中。与对照组相比,灿烂弧菌刺激后,血细胞中 CgPHB2 mRNA 的表达在 6、12 和 24 h 显著上调,CgPHB2 蛋白的丰度在 12-24 h 也有所提高。此外,白芨刺激血细胞12 h后,CgPHB2的绿色信号分别与线粒体和CgLC3的红色信号共定位,且CgPHB2与mtphagy Dye的共定位值明显增加。通过分子对接模拟了 CgPHB2 和 CgLC3 之间的直接相互作用。通过分子对接模拟了CgPHB2和CgLC3之间的直接相互作用,结果表明,在PHB2抑制剂氟唑啉处理的牡蛎中,有丝分裂相关基因的mRNA表达量和有丝分裂比率在白鱀豚刺激后的牡蛎血细胞中明显下降。所有这些结果都表明,CgPHB2 在牡蛎的抗菌免疫反应中参与介导了血细胞的有丝分裂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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