A novel laccase from Trametes polyzona with high performance in the decolorization of textile dyes.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

AMB Express Pub Date : 2024-03-20 DOI:10.1186/s13568-024-01687-3

Daniela Bucchieri, Marco Mangiagalli, Francesca Martani, Pietro Butti, Marina Lotti, Immacolata Serra, Paola Branduardi

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Abstract

Laccases are multicopper oxidases able to oxidize several phenolic compounds and find application in numerous industrial applications. Among laccase producers, white-rot fungi represent a valuable source of multiple isoforms and isoenzymes of these multicopper oxidases. Here we describe the identification, biochemical characterization, and application of laccase 2 from Trametes polyzona (TP-Lac2), a basidiomycete fungus emerged among others that have been screened by plate assay. This enzyme has an optimal temperature of 50 °C and in acidic conditions it is able to oxidize both phenolic and non-phenolic compounds. The ability of TP-Lac2 to decolorize textile dyes was tested in the presence of natural and synthetic mediators at 30 °C and 50 °C. Our results indicate that TP-Lac2 most efficiently decolorizes (decolorization rate > 75%) malachite green oxalate, orange G, amido black10B and bromocresol purple in the presence of acetosyringone and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)-ABTS. Overall, the laccase mediator system consisting of TP-Lac2 and the natural mediator acetosyringone has potential as an environmentally friendly alternative for wastewater treatment in the textile industry.

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一种来自多瘤革霉的新型漆酶，在纺织品染料脱色方面具有很高的性能。

漆酶是一种多铜氧化酶，能够氧化多种酚类化合物，可应用于多种工业领域。在漆酶生产者中，白腐真菌是这些多铜氧化酶的多种异构体和同工酶的宝贵来源。在此，我们介绍了从多瘤革霉（TP-Lac2）中鉴定、生化表征和应用漆酶 2 的情况，TP-Lac2 是一种基生真菌，在平板检测法筛选出的其他真菌中脱颖而出。这种酶的最适温度为 50 °C，在酸性条件下能够氧化酚类和非酚类化合物。在 30 ℃ 和 50 ℃ 的条件下，测试了 TP-Lac2 在天然和合成介质存在的情况下对纺织染料的脱色能力。结果表明，在乙酰丁香酮和 2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）-ABTS 的存在下，TP-Lac2 能最有效地脱色（脱色率大于 75%）孔雀石绿草酸盐、橙 G、氨基黑 10B 和溴甲酚紫。总之，由 TP-Lac2 和天然介质乙酰丁香酮组成的漆酶介质系统有望成为纺织业废水处理的环保型替代品。

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来源期刊

AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

7.20

自引率

2.70%

发文量

141

审稿时长

13 weeks

期刊介绍： AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.