Molecular analyses of exosome-derived miRNAs revealed reduced expression of miR-184-3p and decreased exosome concentration in patients with alveolar echinococcosis

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-13 DOI:10.1016/j.exppara.2024.108734

Ziyan Cui , Wenhao Yu , Zhixin Wang , Fanyu Kong , Gengbo Ye , Jican Yan , Defang Wu , Fei Du , Mingquan Pang , Dalin Shi , Li Ren

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Abstract

Both E. multilocularis and host-derived exosomes are involved in the pathogenic process of alveolar echinococcosis (AE). Exosomes secrete miRNAs that have regulatory roles in host-pathogen interactions in multiple ways. In the present study, we collected and purified supernatants of E. multilocularis cultures, as well as human plasma exosomes. High-throughput sequencing showed the identities of 45 exosomal miRNAs in E. multilocularis. The lengths of these miRNAs ranged from 19 to 25 nucleotides (nt), with the majority (n = 18) measuring 22 nt. Notably, emu-let-7-5p emerged as the most abundant among these miRNAs, with a detected count of 33,097 and also length of 22 nt. Nanoparticle tracking analysis (NTA) showed that the concentration of exosomes in the plasma of AE patients was lower compared to that in the healthy individuals. This result suggested that the concentration of plasma exosomes was able to distinguish AE patients from healthy individuals. Using qRT-PCR to assess the relative expression of 10 miRNAs of E. multilocularis, we showed that the expression of miR-184-3p was downregulated significantly in the exosomes of plasma from AE patients compared to that in the control group. In summary, this study indicates that AE induces a reduction in the concentration of human plasma exosomes, as well as downregulating miR-184-3p in infected individuals.

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外泌体衍生 miRNA 的分子分析表明，肺泡棘球蚴病患者体内 miR-184-3p 的表达减少，外泌体浓度降低。

多角体棘球蚴和宿主衍生的外泌体都参与了肺泡棘球蚴病（AE）的致病过程。外泌体分泌的 miRNA 在宿主与病原体的相互作用中具有多种调控作用。在本研究中，我们收集并纯化了多孢子虫培养上清液以及人体血浆外泌体。高通量测序结果显示，多角孢子虫体内有 45 种外泌体 miRNA。这些 miRNA 的长度从 19 到 25 个核苷酸（nt）不等，其中大多数（n = 18）长度为 22 nt。值得注意的是，emu-let-7-5p 是这些 miRNA 中数量最多的，检测到的数量为 33,097 个，长度也是 22 nt。纳米粒子追踪分析（NTA）显示，AE 患者血浆中外泌体的浓度低于健康人。这一结果表明，血浆中外泌体的浓度能够区分AE患者和健康人。通过 qRT-PCR 评估多角体 10 个 miRNAs 的相对表达，我们发现与对照组相比，AE 患者血浆外泌体中 miR-184-3p 的表达显著下调。总之，这项研究表明，AE 会诱导人体血浆外泌体浓度的降低，并下调感染者体内的 miR-184-3p。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.