Production of Lipid and Xylo-oligosaccharides from Cellulose and Hemicellulose in Reed Sawdust

Abstract

Reed sawdust is a kind of paper mill waste with high cellulose and hemicellulose content. To promote the rational use of resources, it is essential to make full use of waste resources and transform them into new values. In this work, reed sawdust was pretreated with liquid hot water (LHW) at 170 °C for 30 min. A total of 39.00 g/L glucose was obtained after enzymatic saccharification of cellulose at 50 °C, 20 FPU/g-_{reed sawdust} cellulase, 25% (w/v) reed sawdust, in 5 replenishments. When the fermentation was performed 96 h, the medium contained xylo-oligosaccharides (XOS) 11.74 g/L and biomass 15.21 g/L, in which lipid was 4.14 g/L. After spray drying, feed additives containing 29.17% XOS and 10.29% docosahexaenoic acid (DHA) can be prepared. In particular, the hemicellulose and cellulose in reed sawdust are creatively used at the same time without separation, which greatly reduces the cost of purification in traditional processes and provides a new way for the high-value transformation of sawdust resources.