Downregulation of salivary miR-3928 as a potential biomarker in patients with oral squamous cell carcinoma and oral lichen planus

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2024-03-13 DOI:10.1002/cre2.877

Alieh Farshbaf, Farnaz Mohajertehran, Seyed Hamid Aghaee-Bakhtiari, Hossein Ayatollahi, Katayoun Douzandeh, Atessa Pakfetrat, Nooshin Mohtasham

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引用次数: 0

Abstract

Objectives

Recent studies highlighted the role of miR expressed in saliva as reliable diagnostic and prognostic tools in the long-term monitoring of cancer processes such as oral squamous carcinoma (OSCC). Based on a few previous studies, it seems the miR-3928 can be considered a master regulator in carcinogenesis, and it can be therapeutically exploited. This is the first study that compared oral potentially malignant disorder (OLP) and malignant (OSCC) lesions for miR-3928 expression.

Materials and Methods

In this cross-sectional study, saliva samples from 30 healthy control individuals, 30 patients with erosive/atrophic oral lichen planus, and 31 patients with OSCC were collected. The evaluation of miR-3928 expression by q-PCR and its correlation with clinicopathological indices were analyzed by Shapiro−Wilk, Kruskal−Wallis, Pearson's χ², and Mann−Whitney tests. The p-value less than .05 indicated statistically significant results.

Results

Based on nonparametric Kruskal−Wallis test results, there was a statistically significant difference between the ages of three study groups (p < .05). This test demonstrated a statistically significant difference between the average of miR-3928 expression in three study groups (p < .05). The result of the χ² test showed a statistically significant difference in miR-3928 expression between patients with OLP (p = .01) and also patients with OSCC (p < .0001) in comparison to the control group.

Conclusions

The salivary miR-3928 can play a tumor suppressive role in the pathobiology of OSCC, and it is significantly downregulated in patients. According to the potential tumor suppressive role of miR-3928 in the OSCC process, we can consider this microRNA as a biomarker for future early diagnosis, screening, and potential target therapy.

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下调唾液 miR-3928 作为口腔鳞状细胞癌和口腔扁平苔藓患者的潜在生物标记物。

研究目的最近的研究强调了唾液中表达的 miR 在长期监测癌症过程（如口腔鳞状细胞癌（OSCC））中作为可靠的诊断和预后工具的作用。根据以往的一些研究，miR-3928 似乎可被视为致癌过程中的一个主调节因子，并可对其进行治疗。这是第一项比较口腔潜在恶性疾病（OLP）和恶性（OSCC）病变的 miR-3928 表达的研究：在这项横断面研究中，收集了 30 名健康对照者、30 名糜烂性/萎缩性口腔扁平苔藓患者和 31 名 OSCC 患者的唾液样本。研究人员通过 Shapiro-Wilk、Kruskal-Wallis、Pearson's χ2 和 Mann-Whitney 检验分析了 q-PCR 对 miR-3928 表达的评估及其与临床病理指标的相关性。P值小于0.05表示结果具有统计学意义：非参数 Kruskal-Wallis 检验结果显示，三组研究对象的年龄差异有统计学意义（P 2 检验结果显示，OLP 患者 miR-3928 的表达量差异有统计学意义（P = .01），OSCC 患者 miR-3928 的表达量差异也有统计学意义（P 结论：唾液中 miR-3928 的表达量差异有统计学意义（P = .01），OSCC 患者 miR-3928 的表达量差异也有统计学意义（P = .01）：唾液miR-3928可在OSCC的病理生物学中发挥抑瘤作用，且在患者体内明显下调。根据 miR-3928 在 OSCC 过程中的潜在肿瘤抑制作用，我们可以考虑将该微RNA 作为一种生物标记物，用于未来的早期诊断、筛查和潜在的靶向治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.