In vitro Effect of EBV Infection on HERV-K18 env Expression in the Presence and Absence of Vitamin D in Multiple Sclerosis Patients.

Q2 Biochemistry, Genetics and Molecular Biology

Iranian Biomedical Journal Pub Date : 2023-10-29 DOI:10.61186/ibj.3991

Tayebeh Latifi, Majid Teymoori-Rad, Ahmad Nejati, Shohreh Shahmahmoodi, Farhad Rezaei, Sayed Mahdi Marashi

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Abstract

Background: HERV-K env is associated with several neurological disorders, including MS. Clinical studies have demonstrated a plausible interaction between HERV-K env and other MS risk factors. The present study aimed to investigate the possible association between HERV-K18 env and TGF-β. We further assessed the in vitro effect of EBV infection on HERV-K18 env expression in the presence and absence of vitamin D in MS patients.

Methods: PBMCs from 20 MS patients and 20 healthy controls were infected with the B95.8 EBV, seeded into 24-well plates and incubated in the presence or absence of 100 nM of 1,25(OH)D3. The expression levels of HERV-K18 env and TGF-β were measured using real-time PCR.

Results: While the expression level of HERV-K18 env was significantly higher in MS patients than the healthy controls, this trend for TGF-β was significantly reverse. Interestingly, an inverse correlation was found between HERV-K18 env and TGF-β expression in MS patients, although the in vitro stimulation of PBMCs with EBV and vitamin D showed no significant differences in terms of HERV-K18 expression.

Conclusion: Our findings highlight the potential role of HERV-K18 env in MS patients.

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多发性硬化症患者在有维生素 D 和无维生素 D 的情况下 EBV 感染对 HERV-K18 env 表达的体外影响。

背景：HERV-K env 与包括多发性硬化症在内的多种神经系统疾病有关。临床研究表明，HERV-K env 与其他多发性硬化症风险因素之间可能存在相互作用。本研究旨在探讨 HERV-K18 env 与 TGF-β 之间可能存在的关联。我们进一步评估了在有维生素 D 和没有维生素 D 的情况下，EBV 感染对 MS 患者 HERV-K18 env 表达的体外影响：方法：用 B95.8 EBV 感染 20 名多发性硬化症患者和 20 名健康对照者的 PBMC，将其接种到 24 孔板中，在有或无 100 nM 1,25(OH)D3 的情况下进行培养。使用实时 PCR 检测 HERV-K18 env 和 TGF-β 的表达水平：结果：HERV-K18 env 在多发性硬化症患者中的表达水平明显高于健康对照组，而 TGF-β 的表达水平则明显低于健康对照组。有趣的是，尽管用 EBV 和维生素 D 体外刺激 PBMCs 在 HERV-K18 表达方面没有显示出明显差异，但在多发性硬化症患者中 HERV-K18 env 与 TGF-β 表达之间存在反相关性：我们的研究结果凸显了 HERV-K18 env 在多发性硬化症患者中的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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