A universal panel of STR loci for the study of polymorphism of the species Canis lupus and forensic identification of dog and wolf.

IF 0.9 Q3 AGRICULTURE, MULTIDISCIPLINARY
A E Hrebianchuk, I S Tsybovsky
{"title":"A universal panel of STR loci for the study of polymorphism of the species Canis lupus and forensic identification of dog and wolf.","authors":"A E Hrebianchuk, I S Tsybovsky","doi":"10.18699/vjgb-24-12","DOIUrl":null,"url":null,"abstract":"<p><p>Commercial panels of microsatellite (STR) loci are intended for DNA analysis of the domestic dog (Canis lupus familiaris) and, therefore, when genotyping the Grey wolf (Canis lupus lupus), most markers reveal significant deviations from the Hardy-Weinberg equilibrium and have a low informative value, which complicates their use in a forensic examination. The aim of this study was to select STR markers that equally effectively reflect population polymorphism in the wolf and the dog, and to create a universal panel for the identification of individuals in forensic science. Based on the study of polymorphisms of 34 STR loci, a CPlex panel of 15 autosomal loci and two sex loci was developed, which is equally suitable for identifying wolfs and dogs. Analysis of molecular variance (AMOVA) between samples revealed significant differentiation values (FST = 0.0828, p < 0.05), which allows the panel to be used for differentiating between wolf and dog samples. For the first time in the forensic examination of objects of animal origin in the Republic of Belarus, population subdivision coefficients (θ-values) were calculated for each of the 15 STR loci of the test system being reported. It was shown that the values of the genotype frequency, when averaged over all studied animals without and with considering the θ-value, differ by three orders of magnitude (3.39 · 10-17 and 4.71 · 10-14, respectively). The use of population subdivision coefficients will provide the researcher with the most relevant results of an expert identification study. The test system was validated in accordance with the protocol of the Scientific Working Group on DNA Analysis Methods. A computational tool was developed to automate the analysis of genetic data on the wolf and dog in the forensic examination; two guides were approved for practicing forensic experts. This methodology is being successfully used in expert practice in investigating cases of illegal hunting, animal abuse and other offenses in the Republic of Belarus.</p>","PeriodicalId":44339,"journal":{"name":"Vavilovskii Zhurnal Genetiki i Selektsii","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10917668/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vavilovskii Zhurnal Genetiki i Selektsii","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18699/vjgb-24-12","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

Abstract

Commercial panels of microsatellite (STR) loci are intended for DNA analysis of the domestic dog (Canis lupus familiaris) and, therefore, when genotyping the Grey wolf (Canis lupus lupus), most markers reveal significant deviations from the Hardy-Weinberg equilibrium and have a low informative value, which complicates their use in a forensic examination. The aim of this study was to select STR markers that equally effectively reflect population polymorphism in the wolf and the dog, and to create a universal panel for the identification of individuals in forensic science. Based on the study of polymorphisms of 34 STR loci, a CPlex panel of 15 autosomal loci and two sex loci was developed, which is equally suitable for identifying wolfs and dogs. Analysis of molecular variance (AMOVA) between samples revealed significant differentiation values (FST = 0.0828, p < 0.05), which allows the panel to be used for differentiating between wolf and dog samples. For the first time in the forensic examination of objects of animal origin in the Republic of Belarus, population subdivision coefficients (θ-values) were calculated for each of the 15 STR loci of the test system being reported. It was shown that the values of the genotype frequency, when averaged over all studied animals without and with considering the θ-value, differ by three orders of magnitude (3.39 · 10-17 and 4.71 · 10-14, respectively). The use of population subdivision coefficients will provide the researcher with the most relevant results of an expert identification study. The test system was validated in accordance with the protocol of the Scientific Working Group on DNA Analysis Methods. A computational tool was developed to automate the analysis of genetic data on the wolf and dog in the forensic examination; two guides were approved for practicing forensic experts. This methodology is being successfully used in expert practice in investigating cases of illegal hunting, animal abuse and other offenses in the Republic of Belarus.

用于研究狼犬多态性和狗与狼的法医鉴定的通用 STR 位点组。
商业化的微卫星(STR)位点面板主要用于家犬(Canis lupus familiaris)的 DNA 分析,因此在对灰狼(Canis lupus lupus)进行基因分型时,大多数标记都会显示出与哈代-温伯格平衡(Hardy-Weinberg equilibrium)的显著偏差,且信息价值较低,这使其在法医检查中的应用变得复杂。本研究的目的是选择能同样有效地反映狼和狗的种群多态性的 STR 标记,并建立一个用于法医学个体鉴定的通用面板。在对 34 个 STR 位点多态性研究的基础上,开发了一个由 15 个常染色体位点和 2 个性别位点组成的 CPlex 面板,该面板同样适用于识别狼和狗。样本间的分子方差分析(AMOVA)显示了显著的区分值(FST = 0.0828,p < 0.05),这使得该面板可用于区分狼和狗样本。在白俄罗斯共和国对动物源性物品进行法医检验时,首次对报告的检验系统的 15 个 STR 位点中的每个位点计算了种群细分系数(θ 值)。结果表明,在不考虑θ值和考虑θ值的情况下,所有研究动物的基因型频率平均值相差三个数量级(分别为 3.39 - 10-17 和 4.71 - 10-14)。使用种群细分系数将为研究人员提供最相关的专家鉴定研究结果。根据 DNA 分析方法科学工作组的协议,对测试系统进行了验证。开发了一个计算工具,用于在法医检查中自动分析狼和狗的基因数据;批准了两份指南,供执业法医专家使用。这种方法在白俄罗斯共和国调查非法狩猎、虐待动物和其他犯罪案件的专家实践中得到了成功应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Vavilovskii Zhurnal Genetiki i Selektsii
Vavilovskii Zhurnal Genetiki i Selektsii AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
1.90
自引率
0.00%
发文量
119
审稿时长
8 weeks
期刊介绍: The "Vavilov Journal of genetics and breeding" publishes original research and review articles in all key areas of modern plant, animal and human genetics, genomics, bioinformatics and biotechnology. One of the main objectives of the journal is integration of theoretical and applied research in the field of genetics. Special attention is paid to the most topical areas in modern genetics dealing with global concerns such as food security and human health.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信