Quantification of Aging-Related Decreases in Sensory Innervation of the Bladder Trigone in Rats.

IF 1.8 3区 医学 Q3 UROLOGY & NEPHROLOGY
International Neurourology Journal Pub Date : 2024-02-01 Epub Date: 2024-02-29 DOI:10.5213/inj.2346220.110
Mathijs M de Rijk, Saša Peter, Amanda Wolf-Johnston, John Heesakkers, Gommert A van Koeveringe, Lori A Birder
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引用次数: 0

Abstract

Purpose: The prevalence of lower urinary tract symptoms (LUTS), characterized by problems regarding storage and/or voiding of urine, is known to significantly increase with age. Effective communication between the lower urinary tract and the central nervous system (CNS) is essential for the optimal function of this system, and heavily relies on the efficient interaction between the bladder urothelium and the afferent nerve fibers situated in close proximity to the urothelium within the lamina propria.

Methods: We aimed to quantify aging-related differences in the expression of calcitonin gene-related peptide (CGRP, an established marker for sensory nerve fibers) in the trigonal mucosal layers of young (3-4 months) and aged (25-30 months) rats. We evaluated trigonal tissue from 3 animals per age group. Tissue was serially sectioned at 10 μm and stained for CGRP. Images were taken along the full length of the tissue. For each image we computed the total CGRP-positive area (μm2) and the median value for each animal was used for further analysis.

Results: Upon statistical analysis the aged rats show a significantly lower CGRP-positive area compared to young rats (P=0.0049). These results indicate that aging has a negative effect on the area of CGRP-positive signal in the trigone.

Conclusion: The structural and functional integrity of the sensory web in the trigonum of rats is negatively affected by the aging process, potentially leading to impaired communication between the bladder urothelium the CNS. Consequently, these perturbations in the sensory system may contribute to the pathogenesis or exacerbation LUTS.

量化大鼠膀胱三叉神经感觉神经支配随年龄增长而减少的情况
目的:下尿路症状(LUTS)以尿液储存和/或排尿问题为特征,其发病率随着年龄的增长而显著增加。下尿路与中枢神经系统(CNS)之间的有效沟通对该系统的最佳功能至关重要,并在很大程度上依赖于膀胱尿路上皮与位于固有膜内靠近尿路上皮的传入神经纤维之间的有效互动:我们的目的是量化年轻大鼠(3-4 个月)和老龄大鼠(25-30 个月)三叉粘膜层中降钙素基因相关肽(CGRP,一种已确立的感觉神经纤维标记物)表达的衰老相关差异。我们对每个年龄组 3 只大鼠的三叉神经组织进行了评估。对组织进行 10 μm 连续切片,并对 CGRP 进行染色。沿组织全长拍摄图像。我们计算了每张图像的 CGRP 阳性总面积(μm2),并采用每只动物的中值进行进一步分析:经统计分析,老年大鼠的 CGRP 阳性面积明显低于年轻大鼠(P=0.0049)。这些结果表明,衰老对三叉神经中 CGRP 阳性信号的面积有负面影响:结论:大鼠三叉神经感觉网的结构和功能完整性受到衰老过程的负面影响,可能导致膀胱尿路上皮细胞与中枢神经系统之间的交流受损。因此,感觉系统的这些干扰可能会导致尿失禁的发病或加重。
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来源期刊
International Neurourology Journal
International Neurourology Journal UROLOGY & NEPHROLOGY-
CiteScore
4.40
自引率
21.70%
发文量
41
审稿时长
4 weeks
期刊介绍: The International Neurourology Journal (Int Neurourol J, INJ) is a quarterly international journal that publishes high-quality research papers that provide the most significant and promising achievements in the fields of clinical neurourology and fundamental science. Specifically, fundamental science includes the most influential research papers from all fields of science and technology, revolutionizing what physicians and researchers practicing the art of neurourology worldwide know. Thus, we welcome valuable basic research articles to introduce cutting-edge translational research of fundamental sciences to clinical neurourology. In the editorials, urologists will present their perspectives on these articles. The original mission statement of the INJ was published on October 12, 1997. INJ provides authors a fast review of their work and makes a decision in an average of three to four weeks of receiving submissions. If accepted, articles are posted online in fully citable form. Supplementary issues will be published interim to quarterlies, as necessary, to fully allow berth to accept and publish relevant articles.
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