Y. Lamekina, T. Seviarynchyk, E. Ulashchik, T. P. Akhlamionok, P. Y. Baryshchyk, B. Ranishenka, V. V. Shmanai
{"title":"Synthesis of thiol-containing DNA-oligonucleotides using a phosphoramidite reagent based on trans -4-hydroxy-L-prolinol","authors":"Y. Lamekina, T. Seviarynchyk, E. Ulashchik, T. P. Akhlamionok, P. Y. Baryshchyk, B. Ranishenka, V. V. Shmanai","doi":"10.29235/1561-8331-2024-60-1-36-44","DOIUrl":null,"url":null,"abstract":"In this work, a new phosphoramidite reagent for the preparation of thiol-modified oligonucleotides was synthesized. Thiol-specific reagents have been used to demonstrate the reactivity of thiol groups. After modification, conjugates were purified via gel-filtration and characterized with HPLC-MS.","PeriodicalId":20798,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Chemical Series","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the National Academy of Sciences of Belarus, Chemical Series","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29235/1561-8331-2024-60-1-36-44","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Chemistry","Score":null,"Total":0}
引用次数: 0
Abstract
In this work, a new phosphoramidite reagent for the preparation of thiol-modified oligonucleotides was synthesized. Thiol-specific reagents have been used to demonstrate the reactivity of thiol groups. After modification, conjugates were purified via gel-filtration and characterized with HPLC-MS.