Synthesis of thiol-containing DNA-oligonucleotides using a phosphoramidite reagent based on trans -4-hydroxy-L-prolinol

Abstract

In this work, a new phosphoramidite reagent for the preparation of thiol-modified oligonucleotides was synthesized. Thiol-specific reagents have been used to demonstrate the reactivity of thiol groups. After modification, conjugates were purified via gel-filtration and characterized with HPLC-MS.