RNF135 promotes cell proliferation and autophagy in lung adenocarcinoma by promoting the phosphorylation of ULK1.

IF 2.5 4区医学 Q3 ALLERGY

Allergologia et immunopathologia Pub Date : 2024-03-01 eCollection Date: 2024-01-01 DOI:10.15586/aei.v52i2.1048

Lichun Zhuang, Guanhui Shi, Yuejun Sun

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引用次数: 0

Abstract

Objective: To detect the expression of RING finger protein 135 (RNF135) in lung adenocarcinoma tissues and explore its role in the progression of lung adenocarcinoma.

Methods: Bioinformation analysis, quantitative polymerase chain reaction, and immunoblotting technique discovered the expression of RNF135 in lung adenocarcinoma tissues. Cell counting kit-8 and colony formation, immunostaining, and immunoblot assays examined the effects of RNF135 on cell growth and autophagy. Co-immunoprecipitation (Co-IP), immunostaining, and immuoblotting were conducted to confirm the mechanism.

Results: RNF135 was highly expressed in lung adenocarcinoma. In addition, RNF135 promoted lung adenocarcinoma cell growth. Further, data confirmed that RNF135 promoted autophagy in lung adenocarcinoma cells. Mechanically, RNF135 directly interacted with Unc-51-like autophagy activating kinase 1 (ULK1) to promote its phosphorylation level.

Conclusion: RNF135 promoted cell growth and autophagy in lung adenocarcinoma by promoting the phosphorylation of ULK1.

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RNF135 通过促进 ULK1 的磷酸化来促进肺腺癌的细胞增殖和自噬。

目的检测RING指蛋白135（RNF135）在肺腺癌组织中的表达，并探讨其在肺腺癌进展中的作用：生物信息分析、定量聚合酶链反应和免疫印迹技术发现了RNF135在肺腺癌组织中的表达。细胞计数试剂盒-8和集落形成、免疫染色和免疫印迹检测了RNF135对细胞生长和自噬的影响。为了证实其作用机制，还进行了共免疫沉淀（Co-IP）、免疫染色和免疫印迹分析：结果：RNF135在肺腺癌中高表达。此外，RNF135 还能促进肺腺癌细胞的生长。此外，数据证实 RNF135 促进了肺腺癌细胞的自噬。在机制上，RNF135直接与Unc-51样自噬激活激酶1（ULK1）相互作用，促进其磷酸化水平：结论：RNF135通过促进ULK1的磷酸化来促进肺腺癌细胞的生长和自噬。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.