Identification of core gene in chronic rhinosinusitis with nasal polyps and correlations with inflammation-related genes

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology Pub Date : 2024-03-01 DOI:10.1016/j.bjorl.2024.101410

Jingpu Yang , Chang Liu , Jinzhang Cheng , Yunmeng Wang , Zonggui Wang , Wei Zhong

{"title":"Identification of core gene in chronic rhinosinusitis with nasal polyps and correlations with inflammation-related genes","authors":"Jingpu Yang , Chang Liu , Jinzhang Cheng , Yunmeng Wang , Zonggui Wang , Wei Zhong","doi":"10.1016/j.bjorl.2024.101410","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Our aim in this study is to identify the core genes of chronic rhinosinusitis with nasal polyps and analyze the correlations between it and inflammation-related genes.</p></div><div><h3>Methods</h3><p>GSE72713 dataset containing gene expression data of ECRSwNP, nonECRSwNP and healthy samples was obtained from Gene Expression Omnibus (GEO) and filtered by limma to identify DEGs among three groups, then the functions and correlated pathways of DEGs were analyzed using GO and KEGG. The core DEGs were selected by the intersection of DEGs and the PPI network was constructed via STRING. The correlations between the expression levels of CRSwNP core gene and inflammation-related genes were analyzed via the Mann-Whitney <em>U</em> test.</p></div><div><h3>Results</h3><p>The DEGs among ECRSwNP, nonECRSwNP, and CTRL were filtered respectively, and enrichment analysis showed they were associated with olfaction and/or immune responses. The PPI network was constructed by 7 core DEGs obtained via the intersection among three groups, and ALOX15 was confirmed as the core gene in the network. Subsequently, the correlations between the expression levels of ALOX15 and inflammation-related genes were illustrated.</p></div><div><h3>Conclusion</h3><p>In this study, the core gene ALOX15 was selected from the DEGs among ECRSwNP, nonECRSwNP, and CTRL. IL5, IL1RL1, and IL1RAP were found to exhibit a significant positive correlation with ALOX15.</p></div><div><h3>Level of Evidence</h3><p>Level 3.</p></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"90 3","pages":"Article 101410"},"PeriodicalIF":1.7000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1808869424000259/pdfft?md5=9683e7d9e2a10845d8ae47076f3bfe2d&pid=1-s2.0-S1808869424000259-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Otorhinolaryngology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1808869424000259","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OTORHINOLARYNGOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

Our aim in this study is to identify the core genes of chronic rhinosinusitis with nasal polyps and analyze the correlations between it and inflammation-related genes.

Methods

GSE72713 dataset containing gene expression data of ECRSwNP, nonECRSwNP and healthy samples was obtained from Gene Expression Omnibus (GEO) and filtered by limma to identify DEGs among three groups, then the functions and correlated pathways of DEGs were analyzed using GO and KEGG. The core DEGs were selected by the intersection of DEGs and the PPI network was constructed via STRING. The correlations between the expression levels of CRSwNP core gene and inflammation-related genes were analyzed via the Mann-Whitney U test.

Results

The DEGs among ECRSwNP, nonECRSwNP, and CTRL were filtered respectively, and enrichment analysis showed they were associated with olfaction and/or immune responses. The PPI network was constructed by 7 core DEGs obtained via the intersection among three groups, and ALOX15 was confirmed as the core gene in the network. Subsequently, the correlations between the expression levels of ALOX15 and inflammation-related genes were illustrated.

Conclusion

In this study, the core gene ALOX15 was selected from the DEGs among ECRSwNP, nonECRSwNP, and CTRL. IL5, IL1RL1, and IL1RAP were found to exhibit a significant positive correlation with ALOX15.

Level of Evidence

Level 3.

查看原文本刊更多论文

慢性鼻炎伴鼻息肉核心基因的鉴定及其与炎症相关基因的关联

本研究旨在确定慢性鼻炎伴鼻息肉的核心基因，并分析其与炎症相关基因之间的关联。研究人员从基因表达总库（Gene Expression Omnibus，GEO）中获得了包含ECRSwNP、非ECRSwNP和健康样本基因表达数据的GSE72713数据集，并通过limma筛选出了三组样本中的DEGs，然后利用GO、KEGG分析了DEGs的功能和相关通路。通过DEGs的交叉筛选出核心DEGs，并通过STRING构建PPI网络。通过 Mann-Whitney 检验分析了 CRSwNP 核心基因与炎症相关基因表达水平的相关性。分别过滤了ECRSwNP、非ECRSwNP和CTRL中的DEGs，富集分析表明它们与嗅觉和/或免疫反应相关。通过三组交叉得到的 7 个核心 DEGs 构建了 PPI 网络，并确认 ALOX15 为网络中的核心基因。随后，ALOX15的表达水平与炎症相关基因之间的相关性也得到了说明。本研究从 ECRSwNP、nonECRSwNP 和 CTRL 的 DEGs 中选出了核心基因 ALOX15。研究发现，IL5、IL1RL1 和 IL1RAP 与 ALOX15 呈显著正相关。第 3 层

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Brazilian Journal of Otorhinolaryngology OTORHINOLARYNGOLOGY-

CiteScore

3.00

自引率

0.00%

发文量

205

审稿时长

4-8 weeks

期刊介绍： Brazilian Journal of Otorhinolaryngology publishes original contributions in otolaryngology and the associated areas (cranio-maxillo-facial surgery and phoniatrics). The aim of this journal is the national and international divulgation of the scientific production interesting to the otolaryngology, as well as the discussion, in editorials, of subjects of scientific, academic and professional relevance. The Brazilian Journal of Otorhinolaryngology is born from the Revista Brasileira de Otorrinolaringologia, of which it is the English version, created and indexed by MEDLINE in 2005. It is the official scientific publication of the Brazilian Association of Otolaryngology and Cervicofacial Surgery. Its abbreviated title is Braz J Otorhinolaryngol., which should be used in bibliographies, footnotes and bibliographical references and strips.