Molecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of Türkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1

IF 2 3区农林科学 Q4 IMMUNOLOGY

Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2024-03-01 DOI:10.1016/j.cimid.2024.102154

Ufuk Erol , Omer Faruk Sahin , Osman Furkan Urhan , Ahmet Duran Atas , Kursat Altay

{"title":"Molecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of Türkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1","authors":"Ufuk Erol , Omer Faruk Sahin , Osman Furkan Urhan , Ahmet Duran Atas , Kursat Altay","doi":"10.1016/j.cimid.2024.102154","DOIUrl":null,"url":null,"abstract":"<div><p><em>Anaplasma phagocytophilum</em> is a vector-borne zoonotic pathogen and can infect various vertebrate hosts, especially cattle, sheep, goats, horses, and dogs. Molecular-based studies have revealed that the agent has a high genetic diversity and closely related strains circulate in hosts. In this study, 618 sheep blood samples obtained from different geographic regions of Türkiye were researched for <em>A.</em>phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum–like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate among hosts in Türkiye and around the world. <em>A.</em>phagocytophilum-related strains have been reported in molecular-based studies over the last few years, but there is a lack of data on the vector competence, epidemiology, clinical symptoms, and genetic diversity of these pathogens. Therefore, large-scale molecular studies are still needed to obtain detailed data on the above-mentioned topics.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102154"},"PeriodicalIF":2.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Immunology Microbiology and Infectious Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147957124000316","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Anaplasma phagocytophilum is a vector-borne zoonotic pathogen and can infect various vertebrate hosts, especially cattle, sheep, goats, horses, and dogs. Molecular-based studies have revealed that the agent has a high genetic diversity and closely related strains circulate in hosts. In this study, 618 sheep blood samples obtained from different geographic regions of Türkiye were researched for A.phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum–like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate among hosts in Türkiye and around the world. A.phagocytophilum-related strains have been reported in molecular-based studies over the last few years, but there is a lack of data on the vector competence, epidemiology, clinical symptoms, and genetic diversity of these pathogens. Therefore, large-scale molecular studies are still needed to obtain detailed data on the above-mentioned topics.

查看原文本刊更多论文

图尔基耶不同地区羊群中噬细胞无形体及相关菌株的分子调查；附噬细胞无形体-同类1的系统发育分析说明

噬细胞无丝疟原虫是一种媒介传播的人畜共患病原体，可感染各种脊椎动物宿主，尤其是牛、绵羊、山羊、马和狗。基于分子的研究发现，该病原体具有高度的遗传多样性，并且在宿主体内存在近缘菌株。本研究通过 PCR、RFLP 和 DNA 序列分析，对从土耳其不同地区获得的 618 份绵羊血液样本进行了噬细胞甲虫及相关菌株的研究。在 110 份（17.79%）样本中检测到了这些病原体的 DNA。RFLP 分析表明，所有阳性样本都感染了噬菌体样 1，而没有检测到噬菌体样 2 和噬菌体。对随机抽取的 7 个阳性样本的 16 S rRNA 基因进行了部分测序。对这些分离物的系统进化分析表明，至少有两种噬细胞嗜血杆菌样 1 分离物在土耳其和世界各地的宿主中流行。过去几年的分子研究报告了与噬菌体相关的菌株，但缺乏有关这些病原体的病媒能力、流行病学、临床症状和遗传多样性的数据。因此，仍需进行大规模的分子研究，以获得有关上述主题的详细数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Comparative Immunology Microbiology and Infectious Diseases 医学-免疫学

CiteScore

4.60

自引率

0.00%

发文量

102

审稿时长

40 days

期刊介绍： Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.