Investigating Protein-Protein Interactions of Autophagy-Involved TNIP1.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-03-06 DOI:10.1007/7651_2024_525

Michael L Samulevich, Liam E Carman, Brian J Aneskievich

引用次数: 0

Abstract

Myriad proteins are involved in the process of autophagy, which they participate in via their protein-protein interactions (PPI). Herein we outline a methodology for examining such interactions utilizing the case of intrinsically disordered protein (IDP) TNIP1 and its interaction with linear M1-linked polyubiquitin. This includes methods for recombinant production, purification, immuno-identification, and analysis of an IDP associated with autophagy, its ordered binding partner, and means of quantitatively analyzing their interaction.

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调查自噬参与的 TNIP1 蛋白质与蛋白质之间的相互作用

许多蛋白质都参与了自噬过程，它们通过蛋白质-蛋白质相互作用（PPI）参与自噬过程。在本文中，我们以内在紊乱蛋白（IDP）TNIP1 及其与线性 M1 连接的多泛素的相互作用为例，概述了研究这种相互作用的方法。这包括重组生产、纯化、免疫鉴定和分析与自噬相关的 IDP 及其有序结合伙伴的方法，以及定量分析它们相互作用的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.