Rosetta gen. nov. (Chlorophyta): Resolving the identity of red snow algal rosettes

IF 2.8 3区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Journal of Phycology Pub Date : 2024-03-04 DOI:10.1111/jpy.13438

Casey B. Engstrom, Breanna B. Raymond, Joud Albeitshawish, Anastasia Bogdanovic, Lynne M. Quarmby

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Abstract

Thick-walled rosette-like snow algae were long thought to be a life stage of various other species of snow algae. Rosette-like cells have not been cultured, but by manually isolating cells from 38 field samples in southern British Columbia, we assigned a variety of rosette morphologies to DNA sequence. Phylogenetic analysis of Rubisco large-subunit (rbcL) gene, ribosomal internal transcribed spacer 2 (ITS2) rRNA region, and 18S rRNA gene revealed that the rosette-like cells form a new clade within the phylogroup Chloromonadinia. Based on these data, we designate a new genus, Rosetta, which comprises five novel species: R. castellata, R. floranivea, R. stellaria, R. rubriterra, and R. papavera. In a survey of 762 snow samples from British Columbia, we observed R. floranivea exclusively on snow overlying high-elevation glaciers, whereas R. castellata was observed at lower elevations, near the tree line. The other three species were rarely observed. Spherical red cells enveloped in a thin translucent sac were conspecific with Rosetta, possibly a developmental stage. These results highlight the unexplored diversity among snow algae and emphasize the utility of single-cell isolation to advance the centuries-old problem of disentangling life stages and cryptic species.

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Rosetta gen：解决红雪藻莲座的身份问题。

厚壁莲座状雪藻一直被认为是其他各种雪藻的一个生命阶段。莲座状细胞尚未培养出来，但通过人工从不列颠哥伦比亚省南部的38个野外样本中分离出细胞，我们根据DNA序列确定了各种莲座状形态。对 Rubisco 大亚基（rbcL）基因、核糖体内部转录间隔 2（ITS2）rRNA 区域和 18S rRNA 基因的系统发生学分析表明，莲座状细胞在系统群 Chloromonadinia 中形成了一个新的支系。基于这些数据，我们命名了一个新的属--罗塞塔属，其中包括五个新种：R.castellata、R.floranivea、R.stellaria、R.rubriterra 和 R. papavera。在对不列颠哥伦比亚省的 762 个雪地样本进行的调查中，我们只在高海拔冰川上覆盖的雪地上观察到了 R. floranivea，而在海拔较低的树线附近则观察到了 R. castellata。其他三个物种很少被观察到。球形红细胞包裹在一个薄薄的半透明囊中，与罗塞塔同种，可能是一个发育阶段。这些结果突显了雪藻中尚未被探索的多样性，并强调了单细胞分离技术的实用性，有助于解决数百年来一直存在的生命阶段和隐蔽物种的分离问题。

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来源期刊

Journal of Phycology 生物-海洋与淡水生物学

CiteScore

6.50

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： The Journal of Phycology was founded in 1965 by the Phycological Society of America. All aspects of basic and applied research on algae are included to provide a common medium for the ecologist, physiologist, cell biologist, molecular biologist, morphologist, oceanographer, taxonomist, geneticist, and biochemist. The Journal also welcomes research that emphasizes algal interactions with other organisms and the roles of algae as components of natural ecosystems. All aspects of basic and applied research on algae are included to provide a common medium for the ecologist, physiologist, cell biologist, molecular biologist, morphologist, oceanographer, acquaculturist, systematist, geneticist, and biochemist. The Journal also welcomes research that emphasizes algal interactions with other organisms and the roles of algae as components of natural ecosystems.