Matrix Metalloproteinase 11 is Overexpressed in Various Histotypes of Canine Mammary Tumors

IF 1.2 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

National Academy Science Letters Pub Date : 2024-03-03 DOI:10.1007/s40009-024-01393-5

Shefali Sethi, B. V. Sunil Kumar

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Abstract

Matrix metalloproteinase 11 (MMP-11) belongs to the family of matrix metalloproteinases whose serum level has been reported to be elevated in dogs with mammary tumors. The present investigation focuses on assessing the tissue level expression of MMP-11 in various histotypes of canine mammary tumors (CMT). In this study, 20 CMT tissue paraffin blocks preserved in the laboratory were used. Slides were prepared for histopathological examination and immunohistochemistry. Hyperimmune serum raised against the canine recombinant MMP-11 (rMMP-11) in mice was used to standardize immunohistochemistry to assess expression of MMP-11 protein in various histotypes of CMT. Based on the histopathological examination of hematoxylin and eosin (H&E)-stained tissue sections, the CMT tissues were classified into complex carcinoma, mixed mammary tumor, papillary carcinoma and anaplastic carcinoma. A strong MMP-11 expression was observed in 75% of the histotypes of CMT under study. The findings suggest that MMP-11 could act as molecular marker for the immunohistochemistry-based diagnosis of mammary tumors in dogs.

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基质金属蛋白酶 11 在各种组织类型的犬乳腺肿瘤中过表达

基质金属蛋白酶 11（MMP-11）属于基质金属蛋白酶家族，有报道称患乳腺肿瘤的犬血清中该酶水平升高。本研究的重点是评估 MMP-11 在不同组织类型的犬乳腺肿瘤（CMT）中的组织水平表达。本研究使用了实验室保存的 20 块 CMT 组织石蜡块。制备的切片用于组织病理学检查和免疫组化。使用针对小鼠重组犬 MMP-11（rMMP-11）的超免疫血清来标准化免疫组化，以评估 MMP-11 蛋白在不同组织类型的 CMT 中的表达情况。根据苏木精和伊红（H&E）染色组织切片的组织病理学检查，CMT组织被分为复杂癌、混合型乳腺肿瘤、乳头状癌和无弹性癌。在 75% 的 CMT 组织分型中，都观察到了 MMP-11 的强表达。研究结果表明，MMP-11可作为基于免疫组化方法诊断狗乳腺肿瘤的分子标记物。

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来源期刊

National Academy Science Letters 综合性期刊-综合性期刊

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

12 months

期刊介绍： The National Academy Science Letters is published by the National Academy of Sciences, India, since 1978. The publication of this unique journal was started with a view to give quick and wide publicity to the innovations in all fields of science