Agricultural exposures and DNA damage in PBMC of female farmers measured using the alkaline comet assay.

IF 2.6 3区 医学 Q2 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
P Evenden, Q Vandoolaeghe, Y Lecluse, A C Gac, R Delépée, L B Weiswald, E Boutet-Robinet, M Boulanger, S Bonassi, P Lebailly, M Meryet-Figuière
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引用次数: 0

Abstract

Objective: Several studies investigated the link between agricultural occupational exposures and DNA damage, in an attempt to bring elements of biological plausibility to the increased cancer risk associated with them. However, only a few of these studies focused on females.

Methods: The comet assay was performed on PBMC (Peripheral Blood Mononuclear Cells) samples from 245 females working in open field farming and cattle raising, located in the Normandy area of France. Individual questionnaires on tasks performed were administered at the time of sampling to directly assess exposures. Environmental exposures were issued from a questionnaire assessing the farm productions. Linear regression analyses were done using the DNA damage scores.

Results: Regarding direct exposures, several tasks associated with exposure to potentially harmful chemicals were not associated with DNA damage, but a longer duration of use of herbicide on meadows (p = 0.05) or of cleaning and upkeep of agricultural equipment (p = 0.06) revealed higher DNA damage levels, although the number of exposed women was low. Several indirect and/or environmental exposures were associated with DNA damage in multivariate analyses: a larger surface of meadows (p = 0.006) or the presence of poultry (p = 0.03) was associated with less DNA damage, while the presence of swine (p = 0.01) was associated with higher DNA damage. Smokers and former smokers had less DNA damage than non-smokers (p = 0.0008 and p = 0.03).

Conclusions: We report modified levels of DNA damage for those environmentally exposed to meadows, poultry and pig farming, underlining the need for a better knowledge of the potential health risks experienced by females in this setting.

Abstract Image

使用碱性彗星测定法测量农业暴露与女性农民 PBMC 中 DNA 损伤的关系。
目的:有几项研究调查了农业职业暴露与 DNA 损伤之间的联系,试图为与之相关的癌症风险增加提供生物学上的合理性。然而,这些研究中只有少数侧重于女性:对法国诺曼底地区从事露地耕作和养牛的 245 名女性的 PBMC(外周血单核细胞)样本进行了彗星试验。在采样时,对所从事的工作进行了个人问卷调查,以直接评估暴露情况。环境暴露是通过对农场生产进行评估的问卷调查得出的。使用DNA损伤评分进行线性回归分析:在直接接触方面,与接触潜在有害化学物质有关的几项工作与 DNA 损伤无关,但在草地上使用除草剂(p = 0.05)或清洁和保养农业设备(p = 0.06)的时间越长,DNA 损伤程度越高,尽管接触的妇女人数较少。在多变量分析中,一些间接和/或环境暴露与 DNA 损伤有关:草地面积越大(p = 0.006)或有家禽存在(p = 0.03),DNA 损伤越轻,而有猪存在(p = 0.01),DNA 损伤越重。吸烟者和曾经吸烟者的 DNA 损伤程度低于非吸烟者(p = 0.0008 和 p = 0.03):我们报告了暴露于草地、家禽和养猪环境中的人的 DNA 损伤水平的变化,强调有必要更好地了解女性在这种环境下所经历的潜在健康风险。
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来源期刊
International Archives of Occupational and Environmental Health
International Archives of Occupational and Environmental Health 医学-公共卫生、环境卫生与职业卫生
CiteScore
5.60
自引率
6.70%
发文量
127
审稿时长
3 months
期刊介绍: International Archives of Occupational and Environmental Health publishes Editorials, Review Articles, Original Articles, and Letters to the Editor. It welcomes any manuscripts dealing with occupational or ambient environmental problems, with a special interest in research at the interface of occupational health and clinical medicine. The scope ranges from Biological Monitoring to Dermatology, from Fibers and Dust to Human Toxicology, from Nanomaterials and Ultra-fine Dust to Night- and Shift Work, from Psycho-mental Distress and Burnout to Vibrations. A complete list of topics can be found on the right-hand side under For authors and editors. In addition, all papers should be based on present-day standards and relate to: -Clinical and epidemiological studies on morbidity and mortality -Clinical epidemiological studies on the parameters relevant to the estimation of health risks -Human experimental studies on environmental health effects. Animal experiments are only acceptable if relevant to pathogenic aspects. -Methods for studying the topics mentioned above.
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