High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-01 DOI:10.1262/jrd.2023-085

Katsuma YAMAGA, Satohiro NAKAO, Nobuyuki MIKODA, Jorge Mario SZTEIN, Naomi NAKAGATA, Toru TAKEO

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Abstract

Cold transport of the cauda epididymides is a useful technique for shipping laboratory rat sperm. Cold transport of rat sperm avoids potential risks of microbiological infection, animal escape or death, and animal welfare issues. Previously, we reported that a cold-storage solution containing dimethyl sulfoxide and quercetin maintained the fertility of cold-stored rat sperm. However, cold-stored rat sperm exhibited a decreased fertilization rate after 24-h storage. To recover the fertility of cold-stored sperm, we focused on the effects of bovine serum albumin (BSA), a cholesterol acceptor that induces sperm capacitation. We sought to determine the optimal concentration of BSA in fertilization medium based on the fertility of cold-stored rat sperm. High concentrations of BSA (40 mg/ml) enhanced the fertilization rate of cold-stored rat sperm and maintained sperm fertility for 144 h. Embryos derived from cold-stored and BSA-treated sperm normally developed into pups after embryo transfer. In summary, high BSA concentrations enhanced the fertility of cold-stored rat sperm and prolonged the storage period to 144 h, thereby expanding the transportable region for genetically engineered rats.

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高浓度牛血清白蛋白可提高冷藏大鼠精子的受精能力

冷运附睾尾部是运输实验室大鼠精子的一项有用技术。大鼠精子的冷运避免了微生物感染、动物逃逸或死亡以及动物福利问题等潜在风险。此前，我们曾报道过一种含有二甲基亚砜和槲皮素的冷藏溶液可保持冷藏大鼠精子的生育能力。然而，冷藏大鼠精子在储存 24 小时后受精率下降。为了恢复冷藏精子的受精能力，我们重点研究了牛血清白蛋白（BSA）的作用，它是一种能诱导精子获能的胆固醇接受体。根据冷藏大鼠精子的受精能力，我们试图确定受精培养基中 BSA 的最佳浓度。高浓度 BSA（40 毫克/毫升）提高了冷藏大鼠精子的受精率，并使精子的受精能力维持了 144 小时。总之，高浓度的 BSA 可提高冷藏大鼠精子的受精率，并将储存期延长至 144 小时，从而扩大了基因工程大鼠的可运输区域。图文摘要全尺寸图片

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.