Development of a PMA-LAMP visual detection assay for viable Cronobacter sakazakii

IF 2.5 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

International Journal of Dairy Technology Pub Date : 2024-03-01 DOI:10.1111/1471-0307.13073

Qiming Chen, Yang Yu, Xiaodi Chen, Fangming Tu, Peng Wang, Junyi Huang, Zhanmin Liu

引用次数: 0

Abstract

Cronobacter sakazakii in powdered infant formula (PIF) can infect infants with a mortality rate of up to 80%. In this study, a propidium monoazide–loop-mediated isothermal amplification (PMA-LAMP) assay was developed for fast and cost-effective visual detection of viable C. sakazakii. The detection could be finished in 75 min and the results could be identified by the naked eye. The detection limits of the assay were as low as 1.2 × 10¹ cfu/mL and 1.2 × 10² cfu/mL for gel electrophoresis and naked eyes respectively. Finally, the assay also showed good reliability in the artificially contaminated PIF.

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开发一种 PMA-LAMP 视觉检测法，用于检测存活的阪崎克罗诺杆菌

婴儿配方粉（PIF）中的阪崎链球菌可感染婴儿，死亡率高达 80%。本研究开发了一种丙啶单氮环介等温扩增（PMA-LAMP）检测方法，用于快速、经济地用肉眼检测存活的阪崎肠杆菌。检测可在 75 分钟内完成，检测结果可通过肉眼识别。凝胶电泳和肉眼的检测限分别低至 1.2 × 101 cfu/mL 和 1.2 × 102 cfu/mL。最后，该检测方法在人工污染的 PIF 中也表现出良好的可靠性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Dairy Technology 工程技术-食品科技

CiteScore

7.00

自引率

4.50%

发文量

审稿时长

12 months

期刊介绍： The International Journal of Dairy Technology ranks highly among the leading dairy journals published worldwide, and is the flagship of the Society. As indicated in its title, the journal is international in scope. Published quarterly, International Journal of Dairy Technology contains original papers and review articles covering topics that are at the interface between fundamental dairy research and the practical technological challenges facing the modern dairy industry worldwide. Topics addressed span the full range of dairy technologies, the production of diverse dairy products across the world and the development of dairy ingredients for food applications.