Acquisition of cephalosporin resistance genes blaCTX-M-55, blaCTX-M-65, and blaCMY-2 leads to quinolone resistance in colistin-resistant Escherichia coli harboring mcr-1 gene

IF 1.9 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Food Safety Pub Date : 2024-03-01 DOI:10.1111/jfs.13109

Tatsuya Nakayama, Hien Thi Li, Phong Thanh Ngo, Doan Nguyen Minh Tran, Oanh Thi Hoang Nguyen, Phuong Hoai Hoang, Takahiro Yamaguchi, Michio Jinnai, Phuc Do Nguyen, Chinh Van Dang, Yuko Kumeda, Atsushi Hase

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引用次数: 0

Abstract

Food contamination with plasmid-mediated antibiotic-resistant Escherichia coli has not been thoroughly investigated. This study aimed to clarify the prevalence of colistin-resistant AmpC/extended-spectrum β-lactamase (ESBL)-producing E. coli harbouring mcr (COL-ESBL-EC) and to determine antibiotic resistance by comparison with AmpC/ESBL-producing E. coli (ESBL-EC) and colistin-resistant E. coli harbouring mcr (COL-EC). Sixty chicken meats were tested for COL-ESBL-EC contamination. The result showed that 64 COL-ESBL-EC were isolated from 66.7% of the samples, and compared with ESBL-EC and COL-EC were isolated before. The genotypes of the COL-ESBL-EC showed bla_CTX-M-55/TEM, bla_CTX-M-55, bla_CMY-2, and bla_CTX-M-65 were predominant. The COL-ESBL-EC results showed a similar trend for bla_CTX-M identification to that of ESBL-EC. The mcr was investigated, and mcr-1 was detected in COL-ESBL-EC and COL-EC. Antibiotic susceptibility testing revealed that many strains of COL-ESBL-EC and ESBL-EC were resistant to quinolones, and fosfomycin (FOS). These results suggest that COL-ESBL-EC has simultaneously acquired AmpC/ESBL-related, quinolone, and FOS resistance genes in COL-EC.

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头孢菌素耐药基因 blaCTX-M-55、blaCTX-M-65 和 blaCMY-2 的获得导致携带 mcr-1 基因的耐大肠菌群对喹诺酮类药物产生耐药性

质粒介导的耐抗生素大肠埃希菌对食品污染的影响尚未得到深入研究。本研究旨在明确耐大肠菌素AmpC/广谱β-内酰胺酶（ESBL）产大肠埃希菌（COL-ESBL-EC）的流行情况，并通过与AmpC/ESBL产大肠埃希菌（ESBL-EC）和耐大肠菌素产大肠埃希菌（COL-EC）的比较来确定抗生素耐药性。对 60 块鸡肉进行了 COL-ESBL-EC 污染检测。结果显示，从 66.7% 的样本中分离出了 64 个 COL-ESBL-EC，并与之前分离出的 ESBL-EC 和 COL-EC 进行了比较。COL-ESBL-EC 的基因型以 blaCTX-M-55/TEM、blaCTX-M-55、blaCMY-2 和 blaCTX-M-65 型为主。COL-ESBL-EC 的 blaCTX-M 鉴定结果显示出与 ESBL-EC 相似的趋势。对 mcr 进行了调查，在 COL-ESBL-EC 和 COL-EC 中检测到了 mcr-1。抗生素药敏试验显示，COL-ESBL-EC 和 ESBL-EC 的许多菌株对喹诺酮类药物和磷霉素（FOS）耐药。这些结果表明，COL-ESBL-EC 在 COL-EC 中同时获得了 AmpC/ESBL 相关基因、喹诺酮类药物和 FOS 耐药基因。

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来源期刊

Journal of Food Safety 工程技术-生物工程与应用微生物

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Food Safety emphasizes mechanistic studies involving inhibition, injury, and metabolism of food poisoning microorganisms, as well as the regulation of growth and toxin production in both model systems and complex food substrates. It also focuses on pathogens which cause food-borne illness, helping readers understand the factors affecting the initial detection of parasites, their development, transmission, and methods of control and destruction.