Barcode-style lateral flow immunochromatographic strip for the semi-quantitative detection of ochratoxin A in coffee samples.

IF 2.3 3区农林科学 Q2 CHEMISTRY, APPLIED

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment Pub Date : 2024-04-01 Epub Date: 2024-02-28 DOI:10.1080/19440049.2024.2313115

Luis Alberto Contreras Alvarez, Marcia Daniela Lazo Jara, Fabiana Vasconcelos Campos, Jairo Pinto de Oliveira, Marco César Cunegundes Guimarães

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Abstract

Ochratoxin A (OTA) is a mycotoxin contaminating agricultural products produced by fungi, associated with important toxic effects. Thus, the development of fast, sensitive, and economical approaches for OTA detection is crucial. In this study, a barcode-style lateral flow assay for the semi-quantitative detection of OTA in coffee samples was developed. To achieve this goal, a BSA-OTA complex was immobilized in three test zones to compete with OTA molecules in the sample for binding with anti-OTA antibodies labeled with gold nanoparticles. Different concentrations of OTA in the sample produced distinct colour patterns, allowing semi-quantification of the analyte. The assay exhibited high sensitivity, with a limit of detection of 2.5 µg.L^-1, and high reproducibility, with variation coefficient values between 2% and 13%. Moreover, the colour patterns obtained in the analysis with coffee samples were similar to the results obtained with standard OTA solutions, demonstrating a reliable applicability in real samples.

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用于半定量检测咖啡样品中赭曲霉毒素 A 的条形码式横向流动免疫层析条。

赭曲霉毒素 A（OTA）是由真菌产生的一种污染农产品的霉菌毒素，具有重要的毒性作用。因此，开发快速、灵敏、经济的 OTA 检测方法至关重要。本研究开发了一种条形码式横向流动检测法，用于半定量检测咖啡样品中的 OTA。为实现这一目标，在三个测试区固定了 BSA-OTA 复合物，以与样品中的 OTA 分子竞争与金纳米粒子标记的抗 OTA 抗体的结合。样品中不同浓度的 OTA 会产生不同的颜色模式，从而对分析物进行半定量。该检测方法灵敏度高，检测限为 2.5 µg.L-1，重现性好，变异系数在 2% 到 13% 之间。此外，在分析咖啡样品时获得的颜色模式与使用标准 OTA 溶液获得的结果相似，这表明该方法在实际样品中具有可靠的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

7.40

自引率

6.90%

发文量

136

审稿时长

3 months

期刊介绍： Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.