Bio-efficacy and Persistence of Inert Dust Formulations as Stored-grain Protectants against Sitophilus oryzae (L.)

Ganhewa H.T., Perera A.G.W.U.
{"title":"Bio-efficacy and Persistence of Inert Dust Formulations as Stored-grain Protectants against Sitophilus oryzae (L.)","authors":"Ganhewa H.T., Perera A.G.W.U.","doi":"10.31357/fesympo.v27.7163","DOIUrl":null,"url":null,"abstract":"\n \n \nInert dust formulations of natural origin act as promising alternatives in controlling storage infestations caused by deleterious coleopteran insect pests. In that context, laboratory studies were conducted in order to evaluate the insecticidal activity, progeny inhibition and persistence of three commercially available dust formulations, namely, diatomaceous earth, zeolite and cloisite 20A against Sitophilus oryzae adults, a major coleopteran pest of stored grains, throughout 90 days of storage period. Adulticidal and persistence bioassays were conducted to evaluate the toxicity of binary combinations of inert dusts using their sub-lethal doses towards the test insect for 60 days of storage period. Further, ultrastructural architecture of the test insect pest species was examined via Field Emission Scanning Electron Microscopy (FE-SEM), and Energy Dispersive X-Ray Analysis (EDX) to study the uptake/penetration pattern of inert dust particles through the cuticular layers of target insect pest. All inert dust formulations exhibited very efficacious toxic and progeny inhibition activities and extraordinary mortality percentages irrespective of the inert dust used at the end of initial 30 day-long storage period. Thereafter, mortality percentages gradually declined with the progress of the storage time period, declining in the order of, diatomaceous earth>zeolite>cloisite 20A. All the dust formulations successfully inhibited the progeny production and the lowest average progeny production was recorded at the end of initial storage period. Progeny inhibition decreased with the progress of storage time. Binary combinations of inert dust formulations exhibited additive and antagonistic effects against S. oryzae and similarly, the mortality percentages gradually decreased with the prolongation of the storage period. The FE-SEM and EDX micrographs clearly indicated the presence of dust particles and the distribution of their constituting elements on the cuticular layer of the exposed insect pests with the appearances of abrasions and scratches that may have led to dehydration and eventual death of S. oryzae. Thus, findings of the present study suggest that, naturally-derived diatomaceous earth, zeolite and cloisite 20A can be used as eco-friendly means as stored grain protectants against S. oryzae populations successfully in the storage grain systems. \nKeywords: Sitophilus oryzae, Diatomaceous earth, Cloisite 20A, Zeolite \n \n \n","PeriodicalId":205644,"journal":{"name":"Proceedings of International Forestry and Environment Symposium","volume":"22 22","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of International Forestry and Environment Symposium","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31357/fesympo.v27.7163","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Inert dust formulations of natural origin act as promising alternatives in controlling storage infestations caused by deleterious coleopteran insect pests. In that context, laboratory studies were conducted in order to evaluate the insecticidal activity, progeny inhibition and persistence of three commercially available dust formulations, namely, diatomaceous earth, zeolite and cloisite 20A against Sitophilus oryzae adults, a major coleopteran pest of stored grains, throughout 90 days of storage period. Adulticidal and persistence bioassays were conducted to evaluate the toxicity of binary combinations of inert dusts using their sub-lethal doses towards the test insect for 60 days of storage period. Further, ultrastructural architecture of the test insect pest species was examined via Field Emission Scanning Electron Microscopy (FE-SEM), and Energy Dispersive X-Ray Analysis (EDX) to study the uptake/penetration pattern of inert dust particles through the cuticular layers of target insect pest. All inert dust formulations exhibited very efficacious toxic and progeny inhibition activities and extraordinary mortality percentages irrespective of the inert dust used at the end of initial 30 day-long storage period. Thereafter, mortality percentages gradually declined with the progress of the storage time period, declining in the order of, diatomaceous earth>zeolite>cloisite 20A. All the dust formulations successfully inhibited the progeny production and the lowest average progeny production was recorded at the end of initial storage period. Progeny inhibition decreased with the progress of storage time. Binary combinations of inert dust formulations exhibited additive and antagonistic effects against S. oryzae and similarly, the mortality percentages gradually decreased with the prolongation of the storage period. The FE-SEM and EDX micrographs clearly indicated the presence of dust particles and the distribution of their constituting elements on the cuticular layer of the exposed insect pests with the appearances of abrasions and scratches that may have led to dehydration and eventual death of S. oryzae. Thus, findings of the present study suggest that, naturally-derived diatomaceous earth, zeolite and cloisite 20A can be used as eco-friendly means as stored grain protectants against S. oryzae populations successfully in the storage grain systems. Keywords: Sitophilus oryzae, Diatomaceous earth, Cloisite 20A, Zeolite
惰性粉尘制剂作为储藏谷物保护剂对嗜黑麦镰刀菌(Sitophilus oryzae (L.))的生物效力和持久性
天然惰性粉尘制剂在控制有害鞘翅目害虫引起的储藏虫害方面是一种很有前途的替代品。为此,我们进行了实验室研究,以评估三种市售粉尘制剂(即硅藻土、沸石和苜蓿石 20A)在 90 天储存期内对谷物主要鞘翅目害虫--贮藏谷物中的嗜坐螨成虫--的杀虫活性、后代抑制和持久性。为了评估惰性粉尘二元组合的毒性,在 60 天的贮藏期内使用亚致死剂量对受试昆虫进行了成虫杀灭和持久性生物测定。此外,还通过场发射扫描电子显微镜(FE-SEM)和能量色散 X 射线分析(EDX)检查了测试害虫物种的超微结构,以研究惰性粉尘颗粒在目标害虫角质层中的吸收/渗透模式。在长达 30 天的初始储存期结束时,无论使用哪种惰性粉尘,所有惰性粉尘制剂都表现出非常有效的毒性和后代抑制活性以及超高的死亡率。此后,死亡率随着储存时间的延长逐渐下降,下降顺序依次为硅藻土>沸石>硅藻土 20A。所有粉尘配方都成功地抑制了后代的产生,初始储存期结束时平均后代产生量最低。随着贮藏时间的延长,对后代的抑制作用减弱。惰性粉尘制剂的二元组合对 S. oryzae 具有添加和拮抗作用,同样,死亡率也随着储存时间的延长而逐渐降低。FE-SEM 和 EDX 显微照片清楚地表明,在暴露的害虫的角质层上存在粉尘颗粒及其构成元素的分布,并出现擦伤和划痕,这可能会导致 S. oryzae 脱水并最终死亡。因此,本研究的结果表明,天然提取的硅藻土、沸石和景天20A可作为生态友好型储粮保护剂,成功抵御储粮系统中的害虫种群。关键词镰刀菌 硅藻土 硅藻土 20A 沸石
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信