Characterization of resistance genes and replicon typing in Carbapenem-resistant Klebsiella pneumoniae strains.

IF 4.6 2区医学 Q1 MICROBIOLOGY

Annals of Clinical Microbiology and Antimicrobials Pub Date : 2024-02-24 DOI:10.1186/s12941-024-00672-9

Esin Karaman, Ayşegül Çopur Çiçek, Vicdan Şemen, Fatih Şaban Beriş

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引用次数: 0

Abstract

Objective: In our study, K. pneumoniae strains (non-susceptible to carbapenem) (n = 60) were obtained from various clinical samples from Rize State Hospital between 2015 and 2017 and it is aimed to identify antibiotic resistance genes and replicon typing.

Methods: Antibiotic susceptibility tests of the strains were performed with Kirby-Bauer disk diffusion test and the Vitek-2 automated system (BioMerieux, France). Antibiotic resistance genes and replicon typing was characterized by PCR method.

Results: It was determined that K. pneumaniae isolates were mostly isolated from the samples of the intensive care unit. All of the K. pneumoniae strains examined in this study were found to be ampicillin/sulbactam and ertapenem resistant but colistin susceptible. Amoxacillin/clavulonic acid resistance was detected at 98.14% of strains. The bla_OXA-48 gene was mostly detected in isolates. The most common type of plasmid was I1 and 3 different plasmid types were found in five different strains together.

Conclusion: This study also shows that the distribution of NDM-1 and OXA-48 carbapenemases has increased since the first co-display in Türkiye and that IncHI1 is the first record in our country. This study provides an overview of the major plasmid families occurring in multiple antibiotic-resistant strains of K. pneumoniae. To our knowledge, this study represents the first report of IncHI1 record in Türkiye.

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耐碳青霉烯类肺炎克雷伯氏菌耐药基因的特征和复制子分型。

研究目的在我们的研究中，肺炎克氏菌菌株（对碳青霉烯类无敏感性）（n = 60）来自 2015 年至 2017 年期间里泽州立医院的各种临床样本，旨在鉴定抗生素耐药基因和复制子分型：采用柯比-鲍尔盘扩散试验和Vitek-2自动系统（法国BioMerieux公司）对菌株进行抗生素药敏试验。抗生素耐药基因和复制子分型采用 PCR 方法鉴定：结果表明，肺炎双球菌主要是从重症监护室的样本中分离出来的。本研究中检测的所有肺炎克氏菌菌株均对氨苄西林/舒巴坦和厄他培南耐药，但对可乐定敏感。98.14%的菌株对阿莫西林/克拉维酸耐药。分离菌株中大多检测到 blaOXA-48 基因。最常见的质粒类型是 I1，在 5 株不同的菌株中发现了 3 种不同的质粒类型：本研究还表明，自首次在土耳其共同展示以来，NDM-1 和 OXA-48 碳青霉烯酶的分布有所增加，而 IncHI1 是我国的首个记录。本研究概述了肺炎克氏菌多种抗生素耐药菌株中出现的主要质粒家族。据我们所知，本研究是土耳其首次报道 IncHI1 的记录。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Clinical Microbiology and Antimicrobials MICROBIOLOGY-

CiteScore

8.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.