Enzymatic synthesis of biologically active fructose-based saccharides by Aspergillus niger MK788296 levansucrase

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2024-02-20 DOI:10.1016/j.bcdf.2024.100408

Nermeen H. Elzairy , Faten A. Mostafa , Walaa A. Abdel Wahab , Yasser M. Ragab , Abdelgawad M. Hashem , Mohamed A. Abdel-Naby

{"title":"Enzymatic synthesis of biologically active fructose-based saccharides by Aspergillus niger MK788296 levansucrase","authors":"Nermeen H. Elzairy , Faten A. Mostafa , Walaa A. Abdel Wahab , Yasser M. Ragab , Abdelgawad M. Hashem , Mohamed A. Abdel-Naby","doi":"10.1016/j.bcdf.2024.100408","DOIUrl":null,"url":null,"abstract":"<div>Fructose-based saccharides (FBS) and levans received much attention in recent years because of their diverse biological activities and safety which help in their usability in several medical and pharmaceutical applications. In the present study, FBS and levan were synthesized by Aspergillus niger MK788296 levansucrase. Fifteen fructose-based saccharides were synthesized through the interaction between levansucrase concentration (units), duration of synthesis and substrate (sucrose) concentration through a central composite design (CCD). The synthesized FBS and levans exhibited several biological activities including prebiotic, anti-microbial, fibrinolytic, antioxidant and antitumor activities. The prebiotic effect was emphasized by its promotion effect on the growth of Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus plantarum DMS 20174. They also exerted antimicrobial activity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538. In addition, they exerted prominent antitumor activity against colorectal, liver and breast cancer cell lines.</div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100408"},"PeriodicalIF":0.0000,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioactive Carbohydrates and Dietary Fibre","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212619824000081","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Fructose-based saccharides (FBS) and levans received much attention in recent years because of their diverse biological activities and safety which help in their usability in several medical and pharmaceutical applications. In the present study, FBS and levan were synthesized by Aspergillus niger MK788296 levansucrase. Fifteen fructose-based saccharides were synthesized through the interaction between levansucrase concentration (units), duration of synthesis and substrate (sucrose) concentration through a central composite design (CCD). The synthesized FBS and levans exhibited several biological activities including prebiotic, anti-microbial, fibrinolytic, antioxidant and antitumor activities. The prebiotic effect was emphasized by its promotion effect on the growth of Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus plantarum DMS 20174. They also exerted antimicrobial activity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538. In addition, they exerted prominent antitumor activity against colorectal, liver and breast cancer cell lines.

查看原文本刊更多论文

黑曲霉 MK788296 左旋糖酶酶解合成具有生物活性的果糖基糖

果糖基糖（FBS）和左旋糖近年来备受关注，因为它们具有多种生物活性和安全性，可用于多种医疗和制药用途。在本研究中，黑曲霉 MK788296 列凡琥珀酸酶合成了果糖基糖和列凡。通过中心复合设计（CCD），在左旋琥珀酸酶浓度（单位）、合成持续时间和底物（蔗糖）浓度之间进行交互作用，合成了 15 种果糖类糖。合成的 FBS 和 levans 具有多种生物活性，包括益生元活性、抗微生物活性、纤维蛋白溶解活性、抗氧化活性和抗肿瘤活性。益生元效应主要体现在对动物双歧杆菌 BB-12 亚种和植物乳杆菌 DMS 20174 生长的促进作用上。它们还对大肠杆菌 ATCC 8739 和金黄色葡萄球菌 ATCC 6538 具有抗菌活性。此外，它们还对结肠直肠癌、肝癌和乳腺癌细胞株具有显著的抗肿瘤活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioactive Carbohydrates and Dietary Fibre Agricultural and Biological Sciences-Food Science

CiteScore

6.00

自引率

0.00%

发文量

期刊介绍：