Genetic analysis of influenza A viruses of swine from commercial farms in Serbia.

IF 0.5 4区农林科学 Q4 VETERINARY SCIENCES

Veterinaria italiana Pub Date : 2023-07-31 DOI:10.12834/VetIt.2712.17810.2

Jelena Maksimović Zorić, Vesna Milićević, Ljubiša Veljović, Vladimir Radosavljević, Branislav Kureljušić, Ognjen Stevančević, Chiara Chiapponi

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引用次数: 0

Abstract

Swine influenza presents a very important health and economic issue in pig productions worldwide. Viruses that cause the disease are genetically very diverse but usually belong to the H1N1, H1N2 and H3N2 subtype of influenza A viruses. In this study, we sequenced and analyzed the full genome of viruses detected in swine from seven commercial farms. Through the analysis of the complete sequences of internal gene cassette together with previously characterized HA and NA genes we found three different genotypes amongst five completely sequenced viruses. Two viruses possessed a completely H1avN1 genotype (40%) and belonged to the H1avN1 lineage, which is prevalent in European swine populations. The other three viruses have arisen through the reassortment of the genes of H1avN1 and H1N1pdm09 lineages. In one sample we detected coinfection with viruses of H3N2 subtype with genes of H1avN1, H1N1pdm09 and A/swine/Gent/1/1984-like H3N2 lineages that presents a potential environment for the generation of a triple reassortant virus. The presence of the H1N1pdm09 origin M gene in this sample implies the potential risk of the introduction of these viruses into the human population. Phylogenetic analysis of internal gene cassette revealed slower evolution within genes of H1N1pdm09 lineage than those of H1avN1 lineage.

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塞尔维亚商业农场猪 A 型流感病毒的基因分析。

猪流感是全球养猪业中一个非常重要的健康和经济问题。导致该疾病的病毒在基因上非常多样化，但通常属于甲型流感病毒的 H1N1、H1N2 和 H3N2 亚型。在这项研究中，我们对从七个商业农场的猪身上检测到的病毒进行了全基因组测序和分析。通过分析内部基因盒的完整序列以及之前表征的 HA 和 NA 基因，我们在五种完全测序的病毒中发现了三种不同的基因型。两种病毒拥有完全的 H1avN1 基因型（40%），属于欧洲猪群中流行的 H1avN1 系。另外三种病毒则是通过 H1avN1 和 H1N1pdm09 系的基因重组产生的。在一个样本中，我们检测到带有 H1avN1、H1N1pdm09 和 A/swine/Gent/1/1984-like H3N2 系基因的 H3N2 亚型病毒共感染，这为三重变异病毒的产生提供了潜在环境。该样本中存在 H1N1pdm09 起源的 M 基因，这意味着这些病毒有进入人类的潜在风险。内部基因盒的系统进化分析表明，H1N1pdm09 系基因的进化速度比 H1avN1 系慢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinaria italiana VETERINARY SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.