The antioxidant role of pumpkin (Cucurbita pepo) seed extract against acute reproductive toxicity by uranyl acetate in male rats.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI:10.5455/javar.2023.j720

Ghusoon Abdul Kareem Neamah, Muna Abdul Sahib Alkhfaji, Heba Saleh Shaheed

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引用次数: 0

Abstract

Objective: The main goal of the study was to find whether pumpkin (Cucurbita pepo) extract has any preventive or antioxidant properties against acute uranyl acetate (UA)-induced reproductive cytotoxicity.

Material and methods: Four groups each, including 10 adult male rats, were randomly assigned. (GI): the control group was given 1 ml of purified water orally for 30 days. (GII): Rats were given orally, a single dose of 150 mg/kg b.w. UA (GIII): Rats consumed 40 mg/kg b.w. of pumpkin seed extract (PSE) orally every day for 30 days. (GIV): Rats received a single dose (150 mg/kg b.w.) of UA plus a daily oral dose of PSE (40 mg/kg b.w.) for 30 days. Animal sacrifice was used for oxidative stress and histopathological study.

Results: Showed significantly (p ≥ 0.001) elevated malondialdehyde levels in the GII group (6.19 ± 0.4), while GIII and GIV showed no significant differences. Glutathione peroxidase showed a significant (p ≥ 0.001) decrease (2.55 ± 0.2) in the GII group, while in groups (GIII and GIV), it showed a significant (p ≥ 0.001) increase (4.61 ± 0.16, 4.28 ± 0.032), respectively. The histopathological study for GII groups showed sloughing of epithelial cells lining the seminiferous tubules with a decrease in the number of spermatozoa in some tubules. Many sections revealed hyperplasia of the epithelial cells lining the seminiferous tubules with necrosis. The GIII and GIV groups showed normal histological structures with an increase in spermatogenesis in the testes and epididymis tissues.

Conclusion: We concluded that UA causes oxidative stress and histopathological alterations in the rat reproductive system. Pumpkin extract plays a role in improving the activity of the reproductive system.

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南瓜籽提取物对雄性大鼠乙酸铀急性生殖毒性的抗氧化作用

研究目的研究的主要目的是了解南瓜（Cucurbita pepo）提取物是否对急性醋酸铀（UA）诱导的生殖细胞毒性具有预防或抗氧化作用：随机分为四组，每组包括 10 只成年雄性大鼠。(GI）：对照组口服 1 毫升纯净水，连续 30 天。(GII）：给大鼠口服单剂量 150 毫克/千克体重的 UA（GIII）：大鼠每天口服 40 毫克/千克体重的南瓜籽提取物（PSE），连续 30 天。(GIV）：大鼠接受单剂量（150 毫克/千克体重）UA 和每日口服剂量 PSE（40 毫克/千克体重），持续 30 天。动物牺牲用于氧化应激和组织病理学研究：GII 组丙二醛水平（6.19 ± 0.4）明显升高（p ≥ 0.001），而 GIII 和 GIV 组无明显差异。谷胱甘肽过氧化物酶在 GII 组显著下降（2.55 ± 0.2）（p ≥ 0.001），而在 GIII 组和 GIV 组分别显著上升（4.61 ± 0.16、4.28 ± 0.032）（p ≥ 0.001）。GII 组的组织病理学研究显示，曲细精管上皮细胞脱落，一些精管中的精子数量减少。许多切片显示，曲细精管上皮细胞增生并出现坏死。GIII 组和 GIV 组的组织学结构正常，睾丸和附睾组织的精子生成增加：我们得出结论：UA 会导致大鼠生殖系统氧化应激和组织病理学改变。南瓜提取物在改善生殖系统活性方面发挥了作用。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.