Influence of Hibernation and Dehydration on the Hatching Percentage of Artemia Cyst Collected from Solar Saltpan

Selvaraj Soniya, Michaelbabu Serine Michael, Thavasimuthuchinakan Mahesh, T. Citarasu, Anantharajan, Mary Josaphinepunitha, Thangaswamy Selvaraj, M. Michaelbabu
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Abstract

The brine shrimp Artemia franciscana is an important live feed for fin and shell fish larval culture. Cysts of Artemia are naturally found in a wide variety of harsh environments where they are exposed to different changing environmental conditions. Artemia cysts are also exposed to different hydration/dehydration (H/D) conditions during the post-harvest processing period in the Artemia processing industry. India has enormous quantity of cyst production every year from solar saltpans. But due to the lack of proper post-harvest processing technology, most of the cyst are sold to very low price to larval rearing industries. The aim of the present study was to find out the effect of different rate of dehydration, and different preservation temperatures for enhancing cyst hatchability. The cysts were collected from Thamarikulam saltpan in Kanyakumari District, Tamilnadu, India. The collected cysts were dehydrated to different percentages and preserved at 0º, -10˚ and -20˚C for different durations. The study disclosed that the cyst required 1.3h for full hydration in 30ppt saline water and reached full dehydration at 16h in saturated saline solution. Among the three preservation conditions, the maximum hatching percentage (77.95%) was observed in the cyst that preserved at -20˚C for 400days.Among the different rate of dehydration and hibernation, the 40% dehydrated cyst allowed to hibernate at -20˚C had the maximum            hatching percentage. From this study, it is concluded that the optimum of all dehydration percentage, level of chillness and duration of cold storage had equally influenced in the improvement of cyst quality.
冬眠和脱水对日光盐田采集的蒿甲鱼卵孵化率的影响
卤虫(Artemia franciscana)是鳍鱼和贝类幼鱼养殖的重要活饲料。卤虫的胞囊自然存在于各种恶劣的环境中,暴露于不同的变化环境条件下。在蒿鱼加工业的收获后加工过程中,蒿鱼包囊也会暴露在不同的水化/脱水(H/D)条件下。印度每年从晒盐池中生产大量的囊虫。但由于缺乏适当的收获后处理技术,大部分囊蚴都以极低的价格卖给了幼虫饲养业。本研究旨在找出不同脱水率和不同保存温度对提高囊虫孵化率的影响。囊蚴采集自印度泰米尔纳德邦 Kanyakumari 地区的 Thamarikulam 盐场。采集的孢囊按不同比例脱水,并在 0º、-10˚ 和 -20˚C 温度下保存不同时间。研究表明,囊肿在 30ppt 的盐水中需要 1.3 小时才能完全水化,在饱和盐溶液中需要 16 小时才能完全脱水。在三种保存条件中,在-20˚C下保存400天的孢囊孵化率最高(77.95%)。在不同的脱水和冬眠速度中,40%脱水的孢囊在-20˚C下冬眠的孵化率最高。这项研究得出的结论是,所有脱水率、冬眠程度和冷藏时间的最佳值对提高囊胚质量的影响相同。
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