Effect of shear stress on human umbilical vein endothelial cells: A study using bioreactor

Abstract

Shear stress has a significant impact on human umbilical vein endothelial cells (HUVECs). Endothelial cells line the inner surface of blood vessels, and they play a crucial role in regulating vascular function and maintaining blood vessel integrity. Shear stress is the force exerted by flowing blood on the endothelial cells, and it is an essential biomechanical factor that influences endothelial cell behavior. Understanding the effects of shear stress on HUVECs is essential for studying vascular physiology, identifying potential therapeutic targets for cardiovascular diseases, and designing better vascular implants and devices. The present study provides compelling evidence of the significant impact of shear stress on the maintenance of endothelial cell (EC) phenotype. By investigating the effects of shear stress on human umbilical vein endothelial cells (HUVECs), we have unveiled its role in regulating the expression of pro-thrombotic and anti-thrombotic genes. Our findings demonstrate that shear stress influences key molecular players, such as eNOS (endothelial nitric oxide synthase) and vWF (von Willebrand factor), which are critical for vascular homeostasis and thrombotic balance.