The antibiogram of pus cultures in federal tertiary care hospital, Islamabad and its utility in antimicrobial stewardship

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2024-02-10 DOI:10.18502/ijm.v16i1.14871

Farhan Kursheed, Asraar Tabassum, Umme Farwa, Samia Wazir, Muhammad Shafiq, A. Sheikh

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Abstract

Background and Objectives: Antimicrobial resistance has emerged as a significant global health threat. Infections caused by Multi Drug-Resistant (MDR) bacteria pose formidable challenges in terms of treatment options and patient outcomes. Pus cultures serve as crucial diagnostic tools in identifying the agents responsible for various infections, and their antimicrobial susceptibility patterns which help in establishment of empirical therapy guidelines. This study was conducted to determine the pathogen and its susceptibility pattern from pus cultures and to generate antibiogram in our tertiary care setting. Materials and Methods: It was a cross-sectional study, conducted for a period of six months, from July 2022 to December 2022, in the Pathology Department of Pakistan Institute of Medical Sciences (PIMS). Results: Out of total 2507 samples received, 1242 (49.5%) showed positive culture. Among the 1242 positive samples, 364 were Gram positive cocci (GPCs) and 878 were Gram negative rods (GNRs). Methicillin resistant Staphylococcus aureus (MRSA) was the most common isolate (23%) followed by Klebsiella pneumoniae (22.6%), Pseudomonas aeruginosa (16.9%), Enterobacter spp. (15.5%) and Escherichia coli (14.2%). Vancomycin was found to be highly effective (100%) against MRSA. GPCs were highly susceptible to linezolid (98%) while GNRs showed high level of sensitivity to colistin (96%) and tigecycline (92%). Conclusion: The generation of a local antibiogram specific to the hospital setting is essential to effectively manage infections empirically and preserve the efficacy of existing antibiotics. By implementing antimicrobial stewardship practices based on a better understanding of antibiotic susceptibility patterns, we can contribute to the mitigation of antibiotic resistance and improve patient outcomes.

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伊斯兰堡联邦三级护理医院脓液培养的抗生素图谱及其在抗菌药物管理中的作用

背景和目标：抗菌药耐药性已成为全球健康的重大威胁。耐多药（MDR）细菌引起的感染给治疗方案和患者预后带来了严峻挑战。化脓培养物是重要的诊断工具，可用于确定导致各种感染的病原体及其抗菌药敏感性模式，从而帮助制定经验性治疗指南。本研究旨在确定脓培养物中的病原体及其药敏模式，并在我们的三级医疗机构中生成抗生素图谱。材料与方法：这是一项横断面研究，于 2022 年 7 月至 2022 年 12 月在巴基斯坦医学科学研究所（PIMS）病理科进行，为期 6 个月。研究结果在收到的总共 2507 份样本中，有 1242 份（49.5%）样本的培养结果呈阳性。在这 1242 份阳性样本中，364 份为革兰氏阳性球菌（GPCs），878 份为革兰氏阴性杆菌（GNRs）。耐甲氧西林金黄色葡萄球菌（MRSA）是最常见的分离菌株（23%），其次是肺炎克雷伯菌（22.6%）、铜绿假单胞菌（16.9%）、肠杆菌属（15.5%）和大肠埃希菌（14.2%）。万古霉素对 MRSA 非常有效（100%）。GPCs 对利奈唑胺（98%）高度敏感，而 GNRs 则对可乐定（96%）和替加环素（92%）高度敏感。结论要想有效地根据经验管理感染并保持现有抗生素的疗效，就必须针对医院环境制定本地抗生素图谱。通过在更好地了解抗生素敏感性模式的基础上实施抗菌药物管理实践，我们可以为减轻抗生素耐药性和改善患者预后做出贡献。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.