Quantification of Riboflavin and Thiamine in GI (Geographical Indication) Branded Yogurts Collected from Bogura, Bangladesh Using HPLC Equipped with a Fluorescence Detector

Bangladesh Pharmaceutical Journal Pub Date : 2024-02-11 DOI:10.3329/bpj.v27i1.71155

Md Lemon Mia, Md. Zakir Sultan, Md Ashraf Ali, H. Simol, Md Abdus Salam

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Abstract

Thiamine (vitamin B1) and riboflavin (vitamin B2) are both essential vitamins for human health. Thiamine (vitamin B1) is essential for energy metabolism, nervous system function, carbohydrate metabolism, cardiovascular health, digestion, muscle function, etc. On the other hand, Riboflavin (vitamin B2), which acts as an antioxidant, is essential for energy production, healthy skin, hair and nails, eye health, red blood cell production, nervous system function, etc. Due to its specialty, Bogura's famous yogurt was recognized as a Geographical Indication (GI) on June 26, 2023 by the Directorate of Patents, Designs, and Trademarks (DPDT) under the Ministry of Industry, the Government of the People’s Republic of Bangladesh (Prothom Alo, 2023). The purpose of the study was the quantitative determination of two water-soluble vitamins, thiamine and riboflavin, in the GI branded yogurt and whey (Ghol), collected from Bogura, Bangladesh to ensure health benefits. The methods employed for the quantitative analysis of these vitamins were reversed-phase high performance liquid chromatography (RP-HPLC) equipped with a fluorescence detector. The extraction process involved acid hydrolysis followed by enzymatic hydrolysis with takadiastase enzyme. Chromatographic separation was achieved with a Shimadzu Prominence HPLC system using isocratic elution mode on a C18 column (4.6 mm × 250 mm, 5 μm). The correlation coefficients for thiamine and riboflavin were 0.9975 and 0.9974, respectively. The recovery rates for thiamine and riboflavin were found as 97.70% and 105.40%, respectively. Twenty-two samples were analyzed. Thiamine and riboflavin were found in the range of 0.0647 to 0.1396 mg/100 g and 0.0227 to 0.3749 mg/100 g, respectively. It can be concluded that these yogurts were rich in thiamine and riboflavin. Therefore, these yogurts are beneficial for health. Bangladesh Pharmaceutical Journal 27(1): 59-66, 2024 (January)

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利用配备荧光检测器的高效液相色谱法定量检测孟加拉国博古拉 GI（地理标志）品牌酸奶中的核黄素和硫胺素

硫胺素（维生素 B1）和核黄素（维生素 B2）都是人体健康所必需的维生素。硫胺素（维生素 B1）是能量代谢、神经系统功能、碳水化合物代谢、心血管健康、消化和肌肉功能等所必需的。另一方面，核黄素（维生素 B2）是一种抗氧化剂，对能量生成、健康的皮肤、头发和指甲、眼睛健康、红血球生成、神经系统功能等都至关重要。由于其特殊性，博古拉著名的酸奶于 2023 年 6 月 26 日被孟加拉人民共和国政府工业部专利、外观设计和商标局（DPDT）认定为地理标志产品（Prothom Alo，2023 年）。本研究的目的是定量测定从孟加拉国博古拉采集的 GI 品牌酸奶和乳清（Ghol）中的硫胺素和核黄素这两种水溶性维生素，以确保对健康有益。对这些维生素进行定量分析所采用的方法是配备荧光检测器的反相高效液相色谱法（RP-HPLC）。提取过程包括酸水解，然后用他卡地酸酶进行酶水解。色谱分离采用岛津 Prominence HPLC 系统，使用 C18 色谱柱（4.6 mm × 250 mm，5 μm），采用等度洗脱模式。硫胺素和核黄素的相关系数分别为 0.9975 和 0.9974。硫胺素和核黄素的回收率分别为 97.70% 和 105.40%。对 22 个样品进行了分析。硫胺素和核黄素的含量范围分别为 0.0647 至 0.1396 毫克/100 克和 0.0227 至 0.3749 毫克/100 克。由此可以得出结论，这些酸奶富含硫胺素和核黄素。因此，这些酸奶有益健康：59-66，2024 年（1 月）

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Bangladesh Pharmaceutical Journal

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