Impact of Nicosulfuron on Sperm Quality: Insights into Testicular Cell Apoptosis and NF-κB Signaling Pathway in Mice Testes

IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jianqiu Han, Chen Zhao, Qing Shen, Yalei Qi, Yanjia Zhang, Faisal Raza, Yongmei Li, Hajra Zafar, Tengfei Liu, Juan Tan, Honghui Han, Xueyun Ma
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Abstract

Background:: Nicosulfuron, a widely used herbicide in crops, has raised concerns due to its escalating presence as an environmental pollutant, particularly in soil and water. The potential adverse effects of nicosulfuron on animals, including reproductive toxicity, have garnered attention. Objective:: The study aimed to evaluate the reproductive toxicity of nicosulfuron in male mice. Methods:: Male mice were orally administrated with three different concentration gradients (350, 700, and 1400 mg/kg) of nicosulfuron for 35 days. The investigation delved into sperm quality, testicular structures, and expression of cleaved caspase-3 and NF-κB p65 of the testes. Results:: The finding unveiled a correlation between nicosulfuron exposure and detrimental effects on sperm quality and alteration of testicular structure. Notably, parameters, such as sperm survival rate (SUR) and sperm motility (MOT), exhibited a decline in relation to increasing nicosulfuron dosages. Moreover, in the mice subjected to higher doses of nicosulfuron, elevated expression of cleaved caspase-3 and NF-κB p65 was observed in the testes. Interestingly, we also observed an increase of NF-κB p65 expression in the mice exposed to the nicosulfuron. Conclusion:: Our research revealed that exposure to nicosulfuron resulted in compromised sperm quality and alterations in testicular structure. The correlation between nicosulfuron and apoptosis, especially via the NF-κB pathway, provided significant insights into the mechanisms underpinning these detrimental effects. These findings significantly enhance our comprehension of the potential hazards associated with nicosulfuron exposure and its impacts on the reproductive health of animals.
烟嘧磺隆对精子质量的影响:洞察小鼠睾丸细胞凋亡和 NF-κB 信号通路
背景::烟嘧磺隆是一种广泛应用于农作物的除草剂,由于其作为环境污染物(尤其是在土壤和水中)的存在日益严重,已引起人们的关注。烟嘧磺隆对动物的潜在不利影响(包括生殖毒性)已引起人们的关注。研究目的本研究旨在评估烟嘧磺隆对雄性小鼠的生殖毒性。方法雄性小鼠连续35天口服三种不同浓度梯度(350、700和1400毫克/千克)的烟嘧磺隆。调查内容包括精子质量、睾丸结构以及睾丸中裂解的caspase-3和NF-κB p65的表达。结果研究结果揭示了烟嘧磺隆暴露与精子质量和睾丸结构改变之间的相关性。值得注意的是,随着烟嘧磺隆剂量的增加,精子存活率(SUR)和精子活力(MOT)等参数都出现了下降。此外,在使用较高剂量烟嘧磺隆的小鼠睾丸中,还观察到裂解的caspase-3和NF-κB p65的表达升高。有趣的是,我们还观察到暴露于烟嘧磺隆的小鼠中 NF-κB p65 的表达也有所增加。结论我们的研究表明,接触烟嘧磺隆导致精子质量受损和睾丸结构改变。烟嘧磺隆与细胞凋亡(尤其是通过 NF-κB 通路)之间的相关性为我们深入了解这些有害影响的机制提供了重要依据。这些发现大大提高了我们对与烟嘧磺隆接触相关的潜在危害及其对动物生殖健康影响的理解。
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来源期刊
Current molecular pharmacology
Current molecular pharmacology Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
4.90
自引率
3.70%
发文量
112
期刊介绍: Current Molecular Pharmacology aims to publish the latest developments in cellular and molecular pharmacology with a major emphasis on the mechanism of action of novel drugs under development, innovative pharmacological technologies, cell signaling, transduction pathway analysis, genomics, proteomics, and metabonomics applications to drug action. An additional focus will be the way in which normal biological function is illuminated by knowledge of the action of drugs at the cellular and molecular level. The journal publishes full-length/mini reviews, original research articles and thematic issues on molecular pharmacology. Current Molecular Pharmacology is an essential journal for every scientist who is involved in drug design and discovery, target identification, target validation, preclinical and clinical development of drugs therapeutically useful in human disease.
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