A New Producer of Echinocandins, Ascomycete Coleophoma sp. Isolated from the Lichen Stereocaulon paschale

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-07 DOI:10.1134/s0026261723603792

A. V. Hakobjanyan, R. E. Shcherbatov, T. A. Pankratov

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Abstract

For the first time, phytopathogenic fungi of the genus Coleophoma were detected in the lichen genus Stereocaulon paschale (L.) Hoffm. On agarised nutrient medium, strain NM10F28209 formed compact, dense colonies with black ingrowing mycelium and ashy-grey aerial mycelium. Morphological characteristics and analysis of the ITS region of the gene cluster encoding ribosomal RNAs allowed us to assign the new strain to the genus Coleophoma; the closest relative was Coleophoma cylindrospora (Desm.) Höhn. (1919) with 98.28% of similarity. On prolonged incubation for 20 days, the culture formed a brown pigment that diffused into the agar. Extraction using a chloroform:acetone (1 : 1) mixture resulted in a multi-component preparation exhibiting fungicidal activity against yeasts from the Yarrowia and Candida genera. A compound belonging to the group of echinocandins, for which fungicidal activity has previously been demonstrated, was detected in the complex preparation by thin layer chromatography.

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从地衣 Stereocaulon paschale 中分离出的一种新的棘皮霉素生产者-- Ascomycete Coleophoma sp.

摘要首次在地衣属 Stereocaulon paschale (L.) Hoffm.在琼脂营养培养基上，菌株 NM10F28209 形成了紧凑、致密的菌落，菌丝呈黑色，气生菌丝呈灰白色。通过形态学特征和核糖体 RNAs 编码基因簇 ITS 区域分析，我们将新菌株归入鞘翅目鞘氨醇菌属；最近的亲缘种是鞘氨醇圆柱孢蘑菇（Coleophoma cylindrospora (Desm.) Höhn.（1919 年），亲缘关系为 98.28%。(1919) 的相似度为 98.28%。经过 20 天的长期培养，培养物形成了一种扩散到琼脂中的棕色色素。使用氯仿：丙酮（1:1）混合物进行萃取，得到了一种多组分制剂，该制剂对雅罗菌属和念珠菌属的酵母菌具有杀菌活性。通过薄层色谱法，在复合物制剂中检测到了一种属于棘白菌素类的化合物，该化合物以前曾被证明具有杀菌活性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.